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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #304317
Title: Effects of phytoestrogens on expression of genes regulating growth-related processes in rainbow trout

Author
item Cleveland, Beth
item MANOR, MEGHAN - West Virginia University

Submitted to: International Congress on the Biology of Fish
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2014
Publication Date: 8/2/2014
Citation: Cleveland, B.M., Manor, M.L. 2014. Effects of phytoestrogens on expression of genes regulating growth-related processes in rainbow trout [abstract]. International Congress on the Biology of Fish. Paper No. 16.

Interpretive Summary:

Technical Abstract: Phytoestrogens are plant-derived isoflavones that activate estrogen receptors. Phytoestrogen content of aquafeeds is increasing due to higher inclusion levels of soy and other legumes rich in these compounds. It is unknown whether phytoestrogens affect growth-related processes in a manner similar to the catabolic effects estradiol. Rainbow trout were injected with estradiol and phytoestrogens genistein and daidzein, and regulation of growth- and proteolysis-related gene expression was determined after 24 hr. All compounds increased expression of estrogen receptor alpha-1 and vitellogenin in liver. Estradiol and a high dose of genistein (50 µg/g) produced similar gene expression responses, like down-regulation of the GH/IGF axis and up-regulation of igfbp2b. These treatments also increased expression of FA synthesis and beta-oxidation genes in liver. In muscle, estradiol increased while a low dose of genistein (5 ug/g) decreased expression of fbxo32 (atrogin-1). These data generally suggest dietary phytoestrogens negatively regulate growth-related processes in rainbow trout.