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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #308567
Title: Mapping Fusarium solani and Aphanomyces euteiches root rot resistance and root architecture quantitative trait loci in common bean (Phaseolus vulgaris)

Author
item HAGERTY, C - Oregon State University
item CUESTA-MARCOS, A - Oregon State University
item Cregan, Perry
item Song, Qijian
item MCCLEAN, P - North Dakota State University
item NOFFSINGER, S - Seneca Foods
item MYERS, J - Oregon State University

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/1/2015
Publication Date: 8/17/2015
Citation: Hagerty, C.H., Cuesta-Marcos, A., Cregan, P.B., Song, Q., Mcclean, P.E., Noffsinger, S., Myers, J.R. 2015. Mapping Fusarium solani and Aphanomyces euteiches root rot resistance and root architecture quantitative trait loci in common bean (Phaseolus vulgaris). Crop Science. 55:1969-1977.

Interpretive Summary: Root rot diseases are frequently a problem in both snap bean and dry bean production. A population of 177 progeny from the cross of root rot resistant common bean line RR6950 and root rot susceptible line OSU5446 was developed. The parents and the progeny were evaluated for one root rot pathogen in Wisconsin and a different root rot pathogen in Oregon. The DNA of the parents and progeny was also analyzed with the single nucleotide polymorphism (SNP) DNA markers on the BARCBean6K_3 BeadChip and a genetic map was created with the resulting genetic data. Combining the genetic marker data with the root rot data in a quantitative trait locus (QTL) analysis resulted in the identification of five positions along the 11 sets of bean chromosomes that contained genes or other genetic factors impacting root rot resistance. The SNP DNA markers associated with these resistance factors will allow bean breeders to select progeny from crosses with the root rot resistant bean line RR6950 that contain the genetic factors that provide root rot resistance.

Technical Abstract: Root rot diseases of bean (Phaseolus vulgaris L.) are a constraint to dry and snap bean production. We developed the RR138 RIL mapping population from the cross of OSU5446, a susceptible line that meets current snap bean processing industry standards, and RR6950, a root rot resistant dry bean in the small black market class. We evaluated the RR138 RIL population beginning in the F6 generation for resistance to Fusarium solani root rot in Oregon and Aphanomyces euteiches root rot in Wisconsin. The population was evaluated for a set of root architecture traits at the Oregon location. F. solani root rot resistance was evaluated in three seasons whereas A. euteiches resistance was evaluated in two seasons. The RI population was normally distributed for reaction to both diseases. For each disease, RR6950 was resistant and OSU 5446 was susceptible. We assembled a high-density linkage map using 1,689 Single-Nucleotide Polymorphisms (SNP) from an Illumina 6,000 SNP BARCBean6K_3 Beadchip. The map spanned 1,196 cM, over 11 linkage groups, at a density of 1 SNP per 1.4 cM. Three Quantitative Trait Loci (QTL) associated with A. euteiches were identified, each accounting for 5-15% of the total genetic variation, and two QTL associated with F. solani resistance, accounted for 9 and 22% of the total genetic variance. Two QTL for tap root diameter and one QTL for basal root angle were identified. The QTL for the two diseases mapped to different chromosome locations indicating that separate breeding programs would be required for the two root diseases.