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ARS Home » Pacific West Area » Albany, California » Research » Publications at this Location » Publication #309340
Title: Performance of coupled enzymatic hydrolysis and membrane separation bioreactor for antihypertensive peptides production from Porphyra yezoensis protein

Author
item QU, WENJUAN - Jiangsu University
item MA, HAILE - Jiangsu University
item LI, WEN - Jiangsu University
item Pan, Zhongli
item OWUSU, JOHN - Jiangsu University

Submitted to: Process Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/28/2014
Publication Date: 12/18/2014
Citation: Qu, W., Ma, H., Li, W., Pan, Z., Owusu, J. 2014. Performance of coupled enzymatic hydrolysis and membrane separation bioreactor for antihypertensive peptides production from Porphyra yezoensis protein. Process Biochemistry. 50:245-252.

Interpretive Summary: Porphyra yezoensis, a type of red seaweeds, is a potential source to produce peptides that have a significant effect on the treatment of hypertension patients. The combination of enzymatic hydrolysis and membrane separation for the peptide production from Porphyra yezoensis was studied to maximize cost saving and improve efficiency. The objectives of the research were to study three methods of coupling of enzymatic hydrolysis and membrane separation (CEH-MS) and to compare them with the traditional EH and offline MS method. The results showed that the conversion rate of protein, yield of peptides, output of peptides per unit of enzyme, and antihypertensive activity of peptides from the batch CEH-MS method respectively increased by 43.6%, 43.6%, 7.7%, and 3.9% compared with the traditional method. It was concluded the CEH-MS methods were more efficient than the traditional one to gain high utilization rate of raw material or yield of peptides.

Technical Abstract: To explore more efficient production methods of antihypertensive peptides from Porphyra yezoensis protein, three methods of coupling of enzymatic hydrolysis and membrane separation (CEH-MS) were studied and compared with the traditional EH and offline MS method. The results showed that the conversion rate of protein, yield of peptides, output of peptides per unit of enzyme, and antihypertensive activity of peptides from the batch CEH-MS method respectively increased by 43.6%, 43.6%, 7.7%, and 3.9% compared with the traditional method. Correspondingly, the Km reduced by 49.3% and Kcat increased by 58.4%. Moreover, the operation factors of this method had significant effects on the conversion rate of protein and their optimum values were 0.24 g/L enzyme concentration, temperature of 50ºC, pH 9.0, time of 60 min, pump speed of 300 rpm, and 4.0 g/L substrate concentration. Compared with this method, the method of continuous CEH-MS with water feeding significantly increased the conversion rate of protein by 13.3%, yield of peptides by 13.3%, and output of peptides by 13.4% under the runtime of 300 min and continuously feeding 1140 mL water. Compared with the method of continuous CEH-MS with water feeding, the output of peptides was increased 2.6 times by the method of continuous CEH-MS with substrate feeding under the runtime of 60 min and continuously feeding 1709 mL substrate solution. In general, the three CEH-MS methods were more efficient than the traditional one to gain high utilization rate of raw material or yield of peptides.