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Title: Complete genome sequence of an emerging genotype of tobacco streak virus in the U.S.

Author
item Padmanabhan, Chellappan
item GAO, SHAN - Boyce Thompson Institute
item Li, Rugang
item ZHANG, SHOUAN - University Of Florida
item FEI, ZHANGJUN - Boyce Thompson Institute
item Ling, Kai-Shu

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/28/2014
Publication Date: 11/6/2014
Publication URL: http://DOI:10.1128/genomeA.01138-14
Citation: Padmanabhan, C., Gao, S., Li, R., Zhang, S., Fei, Z., Ling, K. 2014. Complete genome sequence of an emerging genotype of tobacco streak virus in the U.S. Genome Announcements. 2(6):e01138-14.

Interpretive Summary: An emerging disease with unknown etiology was observed on zucchini squash fields in South Florida, U.S. Using deep sequencing analyses, Tobacco streak virus (TSV) with a unique genotype similar to those isolates from India was identified for the first time in the U.S. How this novel genotype of TSV was introduced to the U.S. remains unknown. Accurate virus identification is crucial to formulate strategies in disease diagnosis and management.

Technical Abstract: We report the complete genome sequence of an emerging genotype of Tobacco streak virus (TSV) infecting zucchini squash in Florida (TSV_FL13-07), through deep sequencing of sRNAs and validation by Sanger sequencing. TSV_FL13-07 only shares less than 90% sequence identity in three genomic ribonucleic acids to several known U.S. isolates.