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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #315074
Title: Independent mis-splicing mutations in TaPHS1 causing loss of pre-harvest sprouting (PHS) resistance during wheat domestication

Author
item LIU, SHUBING - Kansas State University
item SEHGAL, SUNISH - South Dakota State University
item LIN, MENG - Kansas State University
item LI, JIARUI - Kansas State University
item TRICK, HAROLD - Kansas State University
item GILL, BIKRAM - Kansas State University
item Bai, Guihua

Submitted to: New Phytologist
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/1/2015
Publication Date: 8/10/2015
Publication URL: https://handle.nal.usda.gov/10113/5454537
Citation: Liu, S., Sehgal, S.K., Lin, M., Li, J., Trick, H., Gill, B., Bai, G. 2015. Independent mis-splicing mutations in TaPHS1 causing loss of pre-harvest sprouting (PHS) resistance during wheat domestication. New Phytologist. 208(3):928-35. doi:10.1111/nph.13489.

Interpretive Summary: Pre-harvest sprouting (PHS) is one of the major constraints for wheat, especially white wheat, production worldwide. Previously, we cloned a gene named TaPHS1 for PHS resistance from chromosome 3AS and found that two nucleotide changes (or mutations) in the gene turned PHS resistant genotypes into PHS-susceptible genotypes. Further analysis of the mutations in the gene in 221 wheat wild progenitor lines, 82 wheat cultivars and 249 landraces from the Fertile Crescent indicated that the two mutations for PHS susceptibility might have arisen independently between T. monococcum and T. aestivum during domestication. PHS assay of wheat progenitor accessions showed that the wild types were highly PHS resistant, while domesticated types gained increased PHS susceptibility during domestication. Two KASP DNA markers were developed based on these mutations and can be used to transfer TaPHS1 in breeding through marker-assisted selection.

Technical Abstract: Pre-harvest sprouting (PHS) is one of the major constraints of wheat production in areas where prolonged rainfall occurs during harvest. TaPHS1 is a gene that regulates PHS resistance in wheat and two causal mutations in the gene result in wheat PHS susceptibility. Two KASP markers were developed based on these mutations, and validated in 82 wheat cultivars with known genotypes. These markers can be used to transfer TaPHS1 in breeding through marker-assisted selection. Screening of 327 accessions of wheat progenitors carrying the A genome using the two KASP markers identified different haplotypes in diploid and tetraploid wheat. Only one T. monococcum accession, however, carries both causal mutations and shows PHS susceptibility. PHS assay of wheat progenitor and wheat landraces from Fertile Crescent accessions showed that the wild types were highly PHS resistant, whereas the domesticated type showed increased PHS susceptibility. The two causal TaPHS1 mutations for PHS susceptibility were involved in wheat domestication and might arise independently between T. monococcum and T. aestivum.