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Title: Genetic diversity and structure in Mamey [P. sapota (Jacq.) H.E. Moore & Stearn] by using microsatellite markers

Author
item MARTINEZ-CASTILLO, JAIME - Centro De Investigación Y Asistencia En Tecnología Y Diseño Del Estado De Jalisco
item Arias De Ares, Renee

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2015
Publication Date: 7/11/2015
Citation: Martinez-Castillo, J., Arias De Ares, R.S. 2015. Genetic diversity and structure in Mamey [P. sapota (Jacq.) H.E. Moore & Stearn] by using microsatellite markers [abstract]. Latin American Symposium: Domestication and management of genetic resources.

Interpretive Summary: In order to enrich the collections in banks of germplasm and to accurately identify accessions collected, it is important to develop molecular tools, particularly for species which genomes have not been sequenced. Pouteria sapota is a tree that not only produces abundant and nutritious fruits, but also its bark, leaves and roots have applications in medicine. We have developed molecular markers that permit to accurately identify the geographic origin of these accessions, and that will be a useful resource in banks of germplasm.

Technical Abstract: The tropical plant Pouteria sapota (Jacq.) is known for its edible fruits that contain unique carotenoids, as well as for the fungitoxic, anti-inflamatory and anti-oxidant activity of extracts from its bark, leaves and roots, though its genetics is unknown. We did high-throughput sequencing of microsatellite-enriched libraries of P. sapota, generated 5223 contig DNA sequences, 1.8 Mbp, developed 368 microsatellites markers and tested them on 31 P. sapota individuals from 11 populations (7 wild, 4 cultivated) from Mexico and Puerto Rico. Gene ontology BLAST analysis of the DNA sequences containing microsatellites that effectively discriminated these populations, showed potential association to physiological functions. Neighbor joining and STRUCTURE analysis grouped individuals according to their geographic origin, with discrimination of populations located as close as 3 km apart. At 62 loci, some alleles were observed in wild and not in cultivated individuals. Genetic diversity was similar in wild and cultivated individuals (HE = 0.32 in wild samples and HE = 0.33 in cultivated samples), and some populations were admixed. We discuss the application of the developed microsatellites for the enrichment of germplasm collections, ancestry and domestication studies.