Skip to main content
ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Commodity Utilization Research » Research » Publications at this Location » Publication #316444
Title: Hormonal regulation of floret closure of rice (Oryza sativa)

Author
item HUANG, YOUMING - Jiangxi Agricultural University
item ZENG, XIAOCHUN - Yichun University
item Cao, Heping

Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/26/2018
Publication Date: 6/7/2018
Citation: Huang, Y., Zeng, X., Cao, H. 2018. Hormonal regulation of floret closure of rice (Oryza sativa). PLOS ONE. 13(6). Article e0198828. https://doi.org/10.1371/journal.pone.0198828.
DOI: https://doi.org/10.1371/journal.pone.0198828

Interpretive Summary: Rice flowering refers to the opening and closing of the floret and takes 6–10 days to complete flowering for the tens-hundreds of florets within the same panicle. The regulation of rice floret opening and closing is very important for hybrid rice seed production. However, little is known about the effects of plant hormones on rice flowering. Our previous studies have shown that jasmonate and methyl jasmonate play remarkable roles in promoting rice floret opening. In this study, we investigated the effects of auxins including indole-3-acidic acid (IAA), indole-3-butyric acid (IBA), 1-naphthalene-acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D) and 3,6-dichloro-2-methoxybenzoic acid (DIC) and abscisic acid (ABA) on floret closure of four fertile and three sterile varieties of rice. The results showed that the percentages of closed florets were significantly lower in plants treated with IAA, IBA, 2,4-D, DIC and NAA and that the durations of floret opening were significantly longer in plants treated with the same auxins. The auxins on floret closure exhibited time- and concentration-dependant effects. The higher concentrations of auxins resulted in higher percentages of closed florets and longer time of floret opening. ABA displayed opposite effects of auxins because it increased the percentages of floret closure and decreased the length of floret opening of rice varieties. Similarly, higher concentrations of ABA resulted in lower percentages of closed florets and shorter time of floret opening. The effective concentrations of auxins and ABA on floret closure of rice were 100-200 mg/mL. The effects of auxins and ABA on floret closure were varied somewhat among rice varieties. Our results showed that auxins delayed but ABA promoted the closure of rice floret regardless of the varieties. This study suggests that auxins and ABA are antagonists on floret closure of rice.

Technical Abstract: Rice flowering refers to the opening and closing of the floret and takes 6–10 days to complete flowering for the tens-hundreds of florets within the same panicle. The regulation of rice floret opening and closing is very important for hybrid rice seed production. However, little is known about the effects of plant hormones on rice flowering. Our previous studies have shown that jasmonate and methyl jasmonate play remarkable roles in promoting rice floret opening. In this study, we investigated the effects of auxins including indole-3-acidic acid (IAA), indole-3-butyric acid (IBA), 1-naphthalene-acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D) and 3,6-dichloro-2-methoxybenzoic acid (DIC) and abscisic acid (ABA) on floret closure of four fertile and three sterile varieties of rice. The results showed that the percentages of closed florets were significantly lower in plants treated with IAA, IBA, 2,4-D, DIC and NAA and that the durations of floret opening were significantly longer in plants treated with the same auxins. The auxins on floret closure exhibited time- and concentration-dependant effects. The higher concentrations of auxins resulted in higher percentages of closed florets and longer time of floret opening. ABA displayed opposite effects of auxins because it increased the percentages of floret closure and decreased the length of floret opening of rice varieties. Similarly, higher concentrations of ABA resulted in lower percentages of closed florets and shorter time of floret opening. The effective concentrations of auxins and ABA on floret closure of rice were 100-200 mg/mL. The effects of auxins and ABA on floret closure were varied somewhat among rice varieties. Our results showed that auxins delayed but ABA promoted the closure of rice floret regardless of the varieties. This study suggests that auxins and ABA are antagonists on floret closure of rice.