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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #317242
Title: Assessment of parasitic fungi for reducing soybean cyst nematode with suppressive soil in soybean fields of northeast China

Author
item SONG, JIE - Chinese Academy Of Sciences
item Li, Shuxian
item WEI, WEI - Chinese Academy Of Sciences
item XU, YANLI - Chinese Academy Of Sciences
item YAO, QIN - Chinese Academy Of Sciences

Submitted to: Acta Agriculture Scandinavica Section B – Soil & Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/14/2017
Publication Date: 8/20/2017
Citation: Song, J., Li, S., Wei, W., Xu, Y., Yao, Q. 2017. Assessment of parasitic fungi for reducing soybean cyst nematode with suppressive soil in soybean fields of northeast China. Acta Agriculture Scandinavica Section B – Soil & Plant Science. 67(8):730-736. https://doi.org/10.1080/09064710.2017.1343377.
DOI: https://doi.org/10.1080/09064710.2017.1343377

Interpretive Summary: Soybean cyst nematode (SCN) is a major yield limiting factor for soybean production in areas of China and other regions of the world. Experiments were conducted to study how suppressive soil, in which certain disease(s) fail to develop, against the soybean cyst nematode in a black soil field with continuous cropping of soybean in Northeast China. The number of SCN was determined in a field with 21 years continuous cropping of soybean (SSSS) and 3 years continuous cropping of soybean (SSSC) followed by 1 year of corn. Compared with the SSSC field, the number of SCN significantly (P < 0.05) declined in SSSS field, along with increased soybean root growth. Different fungi (mold) were isolated from the SSSS field and the eggs and bodies of SCN. In greenhouse experiments with suppressive soil, the number of SCN increased rapidly after the addition of a fungal inhibitor, while adding a bacterial antibiotic to the suppressive soil did not increase the number of SCN. The mass of genomic DNA and the population density of Pochonia chlamydosporia, a parasitic fungus of SCN, were significantly (P < 0.05) higher in SSSS field than in SSSC field, and that of other fungi. The fungus P. chlamydosporia may play an important role in suppressing SCN in this suppressive soil in Northeast China.

Technical Abstract: Experiments were conducted to study how suppressive soil developed against the soybean cyst nematode (SCN) in the black soil (Udic mollisol) fields with continuous cropping of soybean in Northeast China. The population of cysts, eggs and J2 of SCN, Heterodera glycines, were studied in a field with 21 years continuous cropping of soybean (SSSS) and 3 years continuous cropping of soybean followed by 1 year corn (SSSC). Compared with the SSSC field, the densities of SCN significantly (P < 0.05) declined in SSSS field, along with increased soybean root growth. Fusarium equiseti, Pochonia chlamydosporia and Paecilomyces lilacinus were isolated from the SSSS field and cysts, eggs and J2 of SCN. In a soil microcosm with suppressive soil, the population of SCN increased rapidly after the addition of pimaricin (a fungal inhibitor), while adding streptomycin (a bacterial antibiotic) to the suppressive soil did not increase the population of SCN. The mass of genomic DNA and the population density of P. chlamydosporia, a parasitic fungus of H. glycines, were significantly (P < 0.05) higher in SSSS field than in SSSC field, and that of other fungi. The fungus P. chlamydosporia may play an important role in suppressing SCN population in this suppressive soil in Northeast China.