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Title: Ectopic expression of Arabidopsis glutaredoxin gene AtGRXS17 in tomato (Solanum lycopersicum) confers tolerance to chilling stress

Author
item HU, YING - Kansas State University
item WU, QINGYU - Cold Spring Harbor Laboratory
item SPRAGUE, STUART - Kansas State University
item PARK, JUNGEUN - Kansas State University
item OH, MYUNGMIN - Chungbuk National University
item RAJASHEKAR, C.B. - Kansas State University
item KOIWA, HISASHI - Texas A&M University
item Nakata, Paul
item CHENG, NING-HUI - Children'S Nutrition Research Center (CNRC)
item HIRSCHI, KENDAL - Children'S Nutrition Research Center (CNRC)
item WHITE, FRANK - Kansas State University
item PARK, SUNGHUN - Kansas State University

Submitted to: Plant Biology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2014
Publication Date: 7/12/2014
Citation: Hu, Y., Wu, Q., Sprague, S.A., Park, J., Oh, M., Rajashekar, C., Koiwa, H., Nakata, P.A., Cheng, N., Hirschi, K.D., White, F.F., Park, S. 2014. Ectopic expression of Arabidopsis glutaredoxin gene AtGRXS17 in tomato (Solanum lycopersicum) confers tolerance to chilling stress [abstract]. Plant Biology Annual Meeting. Abiotic Stress - Temperature, P04030-A, pg 73.

Interpretive Summary:

Technical Abstract: The monothiol glutaredoxin AtGRXS17 from "Arabidopsis" confers thermotolerance in yeast, "Arabidopsis", and tomato plants. Here, we report that AtGRXS17 also enhances tolerance to chilling stress in tomato and is associated with elevation of antioxidant enzyme activities, which are known to be involved in reactive oxygen species (ROS) scavenging, and modulation of cold-responsive components. GFP-AtGRXS17 fusion proteins, which were initially localized in the cytoplasm, migrated into the nucleus during chilling stress. "AtGRXS17"-expressing tomato plants displayed robust growth, altered temporal expression patterns of the endogenous C-repeat/DRE Binding Factor 1 ("SlCBF1") gene, showed dramatic increases in the ROS scavenging enzyme activities and total soluble sugar content, and accumulated significantly less ROS compared to wild-type plants under chilling stress. At the same time, proline concentrations remained unchanged relative to wild-type plants. "AtGRXS17"-expressing plants also displayed longer primary roots than wild-type plants after oxidative stress treatment, indicating that AtGRXS17 may function in chilling tolerance by ameliorating oxidative damage. The findings demonstrate that ectopic expression of "AtGRXS17" provides improved tolerance to chilling stress and may impact multiple abiotic stress responses in important crop species.