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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Publications at this Location » Publication #319586
Title: Development of SSR markers and assessment of genetic diversity of alzuki bean in the Chinese germplasm collection

Author
item CHEN, HONGLIN - National Institute Of Crop Science - Japan
item LIU, LIGING - China Agricultural University
item BAI, PENG - National Institute Of Crop Science - Japan
item WANG, LIXIA - National Institute Of Crop Science - Japan
item WANG, SUHUA - National Institute Of Crop Science - Japan
item Wang, Ming
item CHENG, XUZHEN - National Institute Of Crop Science - Japan

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/15/2015
Publication Date: 9/26/2015
Citation: Chen, H., Liu, L., Bai, P., Wang, L., Wang, S., Wang, M.L., Cheng, X. 2015. Development of SSR markers and assessment of genetic diversity of alzuki bean in the Chinese germplasm collection. Molecular Breeding. 35:191.

Interpretive Summary: Adzuki bean is an important food legume crop in East Asia. China is ranked as the number one country for its production and consumption. A large number of adzuki bean accessions are maintained in the Chinese national seed genebank. Tapping its genetic diversity may have potential in assisting breeding programs for new cultivar development and improvement. However, its genetic diversity was not well evaluated due to lack of sufficient DNA markers. For marker development, we initiated de novo transcriptome sequencing of adzuki bean. From sequence assembly, 65,950 unigenes were identified and some of them were used for eSSR marker development. A set of 53 SSR markers were developed from adzuki bean and 110 SSR (including 57 already available) markers were used for genotyping of 261 selected accessions. These adzuki bean accessions were classified into ten clusters. The accessions from North China were well separated from the accessions from South China. The average polymorphic information content (PIC) of genomic SSR (gSSR) markers and EST-SSR (eSSR) markers was 0.5081 and 0.2382, respectively. Results indicate that gSSR markers were more polymorphic than eSSR markers. These polymorphic eSSR and gSSR markers will be useful for assisting adzuki bean breeding programs and future germplasm curation.

Technical Abstract: Adzuki bean is an important food legume crop in East Asia. China is ranked as the number one country for its production and consumption. A large number of adzuki bean accessions are maintained in the Chinese national seed genebank. Tapping its genetic diversity may have potential in assisting breeding programs for new cultivar development and improvement. However, its genetic diversity was not well evaluated due to lack of sufficient DNA markers. For marker development, we initiated de novo transcriptome sequencing of adzuki bean. From sequence assembly, 65,950 unigenes were identified and some of them were used for eSSR marker development. A set of 53 SSR markers were developed from adzuki bean and 110 SSR (including 57 already available) markers were used for genotyping of 261 selected accessions. These adzuki bean accessions were classified into ten clusters. The accessions from North China were well separated from the accessions from South China. The average polymorphic information content (PIC) of genomic SSR (gSSR) markers and EST-SSR (eSSR) markers was 0.5081 and 0.2382, respectively. Results indicate that gSSR markers were more polymorphic than eSSR markers. These polymorphic eSSR and gSSR markers will be useful for assisting adzuki bean breeding programs and future germplasm curation.