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Title: ANALYSIS OF MONOCLONAL ANTIBODIES REACTIVE WITH THE PORCINE CD2 ANTIGEN

Author
item PESCOVITZ M D - IN UNIV, INDIANA
item AASTED B - ROYAL VET UNIV, DENMARK
item CANALS A - INIA, MADRID, SPAIN
item DOMINGUEZ J - INIA, MADRID, SPAIN
item VIZCAINO J S - INIA, MADRID, SPAIN
item POSPISIL R - PRAGUE, CZECH REPUBLIC
item SINKORA J - PRAGUE, CZECH REPUBLIC
item VALPOTIC I - UNIV ZAGREB, CROATIA
item DAVIS W C - PULLMAN, WA
item Lunney, Joan

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/1/1994
Publication Date: N/A
Citation: N/A

Interpretive Summary: This manuscript summarizes the results of part of the second round of analyses performed by scientists participating in the First International Swine CD Workshop. This workshop was set up to compare and standardize the reactivities of monoclonal antibodies (mAb) that recognize pig leukocyte cell surface markers to assign them specific cluster of differentiation numbers (CD#). For the second round analyses, the reactivities of 54 mAbs on T cell subsets or lines were analyzed by flow cytometry a nd the data subjected to statistical clustering to identify groups of mAbs that reacted similarly with specific pig T cells. These results confirmed the assignment of 5 monoclonal antibodies (mAb) to the CD2 T cell cluster. Another mAb, MSA2, originally clustered with these 5 CD2 specific mAb but was not assigned to a CD grouping because of discordant binding results and the lack of biochemical data. Clustered mAbs were examined in more detail to determine whether they bound to the same area, or epitope, of the CD antigen. Epitope analyses and the molecular weight of the precipitated antigen confirmed that 3 mAb (MSA4, MAC80, MAC83) bound to the same epitope, CD2a, of the swine antigen. Two other mAb (1038-8 and PG168) appear to bind t he same epitope but their antigen molecular weight has not been confirmed; they are noted as reacting with the wCD2a antigen. These results will enable researchers to readily compare results of their studies using porcine CD2 specific mAb and thus enrich swine immunologic studies.

Technical Abstract: The initial results of the First International Swine CD Workshop confirmed the assignment of 5 monoclonal antibodies (mAb) to the CD2 T cell cluster. Another mAb, MSA2, originally clustered with these 5 CD2 specific mAb but was not assigned to any CD grouping because of discordant binding results and the lack of biochemical data. Epitope analyses and the molecular weight of the precipitated antigen confirmed that 3 mAb (MSA4, MAC80, MAC83) bound to the same epitope, CD2a, of the swine antigen. Two other mAb (1038-8 and PG168) appear to bind the same epitope but their antigen molecular weight has not been confirmed; they are noted as reacting with the wCD2a antigen.