Skip to main content
ARS Home » Research » Publications at this Location » Publication #31999
Title: SUMMARY OF FIRST ROUND CLUSTER ANALYSIS: COMPLETE ANTIBODY PANEL

Author
item CARR M M - AFRC, NEWBURY, UK
item Lunney, Joan
item ARN S - MA GEN HOSP, MA
item AASTED B - ROYAL VET UNIV, DENMARK
item BINNS R M - AFRC, CAMBRIDGE, UK
item DAVIS W C - WA STATE UNIV, PULLMAN
item CHOI C - UNIV MN, ST. PAUL, MN
item HOWARD C J - AFRC, NEWBURY, UK
item MISFELDT M - M0 SCH MED, COLUMBIA, MO
item WALKER KAREN - 1265-20-00

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/1/1994
Publication Date: N/A
Citation: N/A

Interpretive Summary: This manuscript summarizes the results of the first round of analyses performed by scientists participating in the First International Swine CD Workshop. This workshop was set up to compare and standardize the reactivities of monoclonal antibodies (mAb) that recognize pig leukocyte cell surface markers. The reactivities of 141 mAbs with 60 target cell types or lines were analyzed by flow cytometry and the data subjected to statistical clustering. The aim of the 1st round cluster analysis was to identify groups of mAbs that reacted similarly with specific pig cells. A restricted number of laboratories collected flow cytometry data for the complete mAb panel on the 60 different target cells. These data were then analyzed using a computer software package developed to identify the human CD antigens. MAbs were assigned to 23 clusters by statistical similarity and information on specificity supplied by the contributor. Clustered mAbs swere examined in more detail in the 2nd round of workshop analyses. The statistically similar clusters of mAbs generated from this first round of data were to be used as guidelines for the 2nd round of the workshop analyses, alongside information supplied by the contributors on mAb specificity. The final mAb specificities and workshop nomenclature were decided after the 2nd round of workshop analyses.

Technical Abstract: The reactivities of 141 mAbs with 60 target cell types or lines were analyzed by flow cytometry and the data subjected to statistical clustering. The aim of the 1st round cluster analysis was to identify groups of mAbs with statistically similar pat terns of reactivities. A restricted number of laboratories collected flow cytometry data for the complete mAb panel on a variety of target cells. These data were analyzed using a computer software package. MAbs were assigned to 23 clusters by statistical similarity and information on specificity supplied by the contributor. Clustered mAbs were examined in more detail in the 2nd round of workshop analyses. The statistically similar clusters of mAbs generated from these data were to be used a s guidelines for the 2nd round of the workshop analyses, alongside information supplied by the contributors on mAb specificity. The final mAb specificities and workshop nomenclature were to be decided after the 2nd round of workshop analyses.