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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Insect Control and Cotton Disease Research » Research » Publications at this Location » Publication #320309
Title: Interactions between a cotton phytopathogen and the host using a genomics analysis

Author
item Medrano, Enrique
item Mantooth, Whitney
item Bell, Alois - Al

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/21/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cotton (Gossypium hirsutum L.) is an economic crop grown worldwide. Numerous G. hirsutum polyploids have been sequenced. Bacterial infections of cotton can cause major yield losses. Pantoea ananatis is a known bacterial pathogen of both cotton buds and bolls. Thus, we conducted a whole genome analysis of the infectious P. ananatis strain CFH 7-1. Strain CFH 7-1 is associated with transmission by the cotton fleahopper (Pseudatomoscelis seriatis). Using Roche technology, a high quality draft of the whole genome was generated for the purpose of finding virulence genes to match respective host avirluence genes from published cotton genomes. Both a chromosome (4.5 Mb) and extrachromosomal DNA (2.5 kb) were identified. Annotation of the bacterial genome (4405 CDS) revealed putative pathogenicity elements including type IV and VI secretion systems. Avirulence genes in the recently published allotetrapliod G. hirsutum acc. TM-1 have been potentially identified. This work assists in developing techniques to minimize damages associated with P. ananatis infections by analyzing both the host and phytopathogen genomes.