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ARS Home » Research » Publications at this Location » Publication #32058
Title: MUSCLE PROTEIN PROFILES OF TRANSGENIC PIGS EXPRESSING A BOVINE GROWTH GENE

Author
item VAN LAACK RIETTE - 1280-15-00
item Solomon, Morse
item Pursel, Vernon

Submitted to: International Congress of Meat Science and Technology Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/7/1994
Publication Date: N/A
Citation: N/A

Interpretive Summary: Protein profiles of the longissimus muscle (LM) from transgenic pigs, expressing a bovine growth hormone gene, and control pigs were investigated. Each transgenic (T) pig was compared with a control (C) pig of similar body weight. Samples were excised within 1 h after slaughter. Sarcoplasmic proteins, e.g., the muscle proteins that are soluble in low salt concentration, and myofibrillar proteins, the proteins that are insoluble in low salt concentration, were separated. The sarcoplasmic and myofibrillar fractions were analyzed by means of SDS-polyacrylamide gel electrophoresis (SDS-PAGE). With this electrophoresis procedure, proteins are separated based upon molecular weight. The percentage of polyacrylamide determines the range of molecular weights that can be separated. Protein profile was assessed by means of SDS-PAGE with 7.5, 12 and 15 percent polyacrylamide. Neither molecular weight nor concentration of the various proteins differed between C- and T-pigs. It is concluded that both the myofibrillar and sarcoplasmic protein profiles of C- and T-pigs were similar.

Technical Abstract: Protein profiles of the longissimus muscles (LM) from transgenic pigs, expressing a bGH gene, and control pigs were investigated. Each transgenic (T) pig was compared with a control (C) pig of similar body weight. Samples were excised within 1 h postmortem. Myofibrillar and sarcoplasmic proteins were separated and the protein profile was assessed by means of SDS-PAGE (7.5, 12 and 15 percent polyacrylamide). Neither molecular weigh nor concentration of the various proteins differed between C- and T-pigs. Both the myofibrillar and sarcoplasmic protein profiles of C- and T-pigs were similar.