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ARS Home » Northeast Area » University Park, Pennsylvania » Pasture Systems & Watershed Management Research » Research » Publications at this Location » Publication #322340
Title: Effect of starchy or fibrous carbohydrate supplementation of an herbage diet on ruminal fermentation and methane output in continuous culture

Author
item Soder, Kathy
item HAFLA, AIMEE - Agri-King, Inc
item BRITO, ANDRE - University Of New Hampshire
item Rubano, Melissa
item Dell, Curtis

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/10/2015
Publication Date: 3/10/2015
Citation: Soder, K.J., Hafla, A., Brito, A., Rubano, M.D., Dell, C.J. 2015. Effect of starchy or fibrous carbohydrate supplementation of an herbage diet on ruminal fermentation and methane output in continuous culture. Proceedings of the Northeast Pasture Consortium Meeting, March 10-11, 2015, Morgantown, WV. p. 1.

Interpretive Summary: An interpretive summary is not required.

Technical Abstract: A 4-unit dual-flow continuous culture fermentor system was used to assess the effect of supplementing 2 levels (5 and 10% of diet DM) of starchy (barley grain: BAR) or fibrous (beet pulp: BP) carbohydrate (CHO) to an orchardgrass diet on nutrient digestibility, VFA production, bacterial protein synthesis, and methane output. Treatments were randomly assigned to fermentors in a 4 x 4 Latin square design with a 2 x 2 factorial arrangement using 7 d for diet adaptation and 3 d for sample collection. Treatments included: 1) 57 g DM herbage + 3 g DM BAR; 2) 54 g DM herbage + 6 g DM BAR; 3) 57 g DM herbage + 3 g DM BP; 4) 54 g DM herbage + 6 g DM BP. Feeding and pH sampling occurred at 0730, 1030, 1400 and 1900 h. Gas samples for methane analysis were collected at 0725, 0900, 1000, 1355, 1530, and 1630 h. Effluent samples were analyzed for OM, CP, NDF, nutrient digestibilities, estimation of bacterial protein synthesis, ammonia-N, and VFA. Data were analyzed using the MIXED procedure of SAS with period and treatment as fixed effects and fermentor as random effect. Orthogonal contrasts were tested for CHO type and level of supplement. No significant interactions were detected. Apparent and true OM digestibilities were not affected (P > 0.10) by CHO source (72.4 and 81.9%, respectively). True CP digestibility was greater (P < 0.05) for BP (75.3%) than BAR (52.5%) diets. Apparent NDF digestibility was lower (P < 0.05) for BP (79.5%) than BAR (85.1%) diets. Barley diets produced lower (P < 0.05) molar proportions of acetate (43.5 vs. 49.4 mol/100 mol, respectively), lower concentrations of total VFA (67.2 vs. 72.2 mmol/L, respectively) and tended (P = 0.08) to have greater mean pH (6.75 vs. 6.72) compared with BP diets. Methane production was not affected (P > 0.10) by CHO source. The 10% supplement produced greater (P < 0.05) concentrations of methane (35 vs. 27 mmol/d) and tended (P = 0.07) to increase apparent DM digestibility. Diet had no effect on bacterial efficiency. Supplementation of an herbage-based diet with BP improved CP digestibility compared with barley but did not affect OM digestibility, methane production or microbial efficiency.