Author
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DEBOFSKY, ABIGAIL - University Of Wisconsin |
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KLINGER, REBEKAH - University Of Wisconsin |
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MORA, FRANCISCO - University Of Wisconsin |
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WALTZ, MARCUS - University Of Wisconsin |
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Shepherd, Brian |
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LARSON, JEREMY - University Of Wisconsin |
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ANDERSON, DAVID - University Of Wisconsin |
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YANG, LUOBIN - Idaho State University |
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GOETZ, FREDERICK - National Oceanic & Atmospheric Administration (NOAA) |
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BASU, NILADRI - McGill University - Canada |
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HEAD, JESSICA - McGill University - Canada |
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TONELLATO, PETER - University Of Wisconsin |
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MURPHY, CHERYL - Michigan State University |
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CARVAN, MICHAEL - University Of Wisconsin |
Submitted to: Society of Environmental Toxicology and Chemistry (SETAC)
Publication Type: Abstract Only Publication Acceptance Date: 10/10/2015 Publication Date: 11/1/2015 Citation: Debofsky, A.R., Klinger, R., Mora, F.X., Waltz, M., Shepherd, B.S., Larson, J., Anderson, D., Yang, L., Goetz, F., Basu, N., Head, J., Tonellato, P., Murphy, C., Carvan, M.J. 2015. Scaling the sublethal effects of methylmercury to yellow perchs population dynamics using adverse outcome pathway framework [abstract]. Society of Environmental Toxicology and Chemistry Abstracts. Poster No. RP081. Interpretive Summary: Technical Abstract: This study sought to evaluate the effects of environmentally relevant dietary MeHg exposures on adult female yellow perch (Perca flavescens) and zebrafish (Danio rerio) reproduction. Yellow perch were used in the study for their socioeconomic and ecological importance within the Great Lakes basin, and the use of zebrafish allowed for a detailed analysis of the molecular effects of MeHg following a whole life-cycle exposure. These zebrafish exposures better mimic realistic wildlife exposure scenarios, and the twenty-week adult yellow perch exposure (where whole life-cycle exposures are difficult) captures early seasonal ovarian development. In zebrafish, several genes involved in reproductive processes were shown to be dysregulated by RNA-seq and QPCR, but no significant phenotypic changes were observed with ovarian staging, fecundity, or embryo mortality. Yellow perch did not appear to be affected by MeHg at the molecular level, as assessed by QPCR of eight genes expressed in the pituitary, liver, and ovary. Unfortunately, a lack of genomic resources in yellow perch hinders the characterization of any subtle molecular impacts. The ovarian somatic index, circulating estradiol, and ovarian staging were not significantly altered by MeHg exposure in yellow perch. These results suggest that environmentally relevant MeHg exposures do not drastically reduce the spawning capacity of these fish, but to capture realistic exposure scenarios, whole life-cycle yellow perch exposures are needed. |