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ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #323217
Title: Laboratory screening of a peanut recombinant inbred line population for aflatoxin resistance

Author
item JI, XIANGYUAN - University Of Georgia
item GUO, XIAOHONG - University Of Georgia
item FOUNTAIN, JAKE - University Of Georgia
item PANDEY, MANISH - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item NAYAK, SPURTHI - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item WANG, HUI - University Of Georgia
item Holbrook, Carl - Corley
item VARSHNEY, RAJEEV - Heilongjiang Bayi Agricultural University (HLAU)
item Guo, Baozhu

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/29/2015
Publication Date: 10/29/2015
Citation: Ji, X., Guo, X., Fountain, J., Pandey, M., Nayak, S.N., Wang, H., Holbrook Jr, C.C., Varshney, R.K., Guo, B. 2015. Laboratory screening of a peanut recombinant inbred line population for aflatoxin resistance [abstract]. University of Georgia Plant Center Retreat Meeting.

Interpretive Summary:

Technical Abstract: Aflatoxin is considered to be serious impediment for crop production in the Southern US resulting from infection by Aspergillus flavus. Aflatoxin contamination is a health concern. To date, the only successful methods of remediating aflatoxin contamination include proper storage conditions for harvested grains, biological controls, and resistant cultivars. In peanut, limited genetic variation is available in breeding programs necessitating the identification of resistant germplasm for use in varietal development. In this study, we screened a 163 recombinant inbred lines (RILs) generated from Tifrunner x GT-C20 for resistance to aflatoxin contamination using a laboratory kernel screening assay. We scored visible sporulation on peanut kernels (1 to 10). Aflatoxin content was also measured using a fast methanol extraction method and a fluorimeter. This study was repeated three times and each time had three bio-replications. We found that 53 lines had 0-30% surface sporulation, 75 lines had 40-70% surface sporulation, and 37 lines had 80-100% surface sporulation indicating a normal distribution of ratings across the population. Aflatoxin contamination levels varied widely among the RILs (0-1149540ppb). Also, a majority of the lines (143) had aflatoxin levels lower than the parental lines indicating skewness in the population toward lower aflatoxin levels. This also indicated that visible sporulation and aflatoxin contamination are not necessarily correlated in kernel screening assays. The QTL analysis will be carried out later, and this study may provide info for resistance to aflatoxin contamination in peanut breeding.