A reference model systesm of industrial yeasts Saccharomyces cerevisiae is needed for development of the next-generation biocatalyst toward advanced biofuels production

Authors: LIU, ZONGLIN; WANG, XU - SICHUAN AGRICULTURAL UNIVERSITY

Submitted to: Journal of Microbial and Biochemical Technology
Publication Type: Other
Publication Acceptance Date: 11/3/2015
Publication Date: 11/3/2015
Publication URL: http://handle.nal.usda.gov/10113/62380
Citation: Liu, Z., Wang, X. 2015. A reference model systesm of industrial yeasts Saccharomyces cerevisiae is needed for development of the next-generation biocatalyst toward advanced biofuels production. Journal of Microbial and Biochemical Technology. 7:6. doi: 10.4172/1948-5948.1000e125

Interpretive Summary: Well-known laboratory model strains of Saccharomyces cerevisiae are commonly used as host strains in research for new strain development. However, performance of such derived new strains is often inconsistent from strains developed from more tolerant industrial yeast strains. Extended practice of the laboratory model strain in such a usage not only confuses literature but also can be misleading because it hinders the efficient development of the next-generation of biocatalysts in a bio-based economy. This research found substantial evidence on performance response between the industrial yeast strains and the laboratory model strains, specifically in the area of the new strain development. It concluded that the haploid model strains are not suitable to be used as host strains for new strain development through genetic engineering efforts. Instead, a well rounded model system for the diploid industrial yeast is needed for efficient development of the next-generation biocatalysts towards production of advanced biofuels and chemicals. This work will benefit academic and industrial research and development communities worldwide for new strain development in industrial applications, and contribute to literature in basic science.

Technical Abstract: Diploid industrial yeast Saccharomyces cerevisiae has demonstrated distinct characteristics that differ from haploid laboratory model strains. However, as a workhorse for a broad range of fermentation-based industrial applications, it was poorly characterized at the genome level. Observations on the haploid model strain performance, particularly as a host strain for new strain development, are often inconsistent with the response of the diploid industrial yeast strains. An industrial yeast model system is urgently needed for efficient development of the next-generation biocatalysts towards a sustainable production of advanced biofuels and chemicals.