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Title: Triclosan in Campy-Cefex Agar to aid in Enumeration of Naturally Occurring Campylobacter spp. in Broiler Ceca

Author
item DENT, CAMILLE - University Of Georgia
item Berrang, Mark
item Adams, Eric
item Meinersmann, Richard - Rick

Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: 11/16/2015
Publication Date: 1/25/2016
Citation: Dent, C.I., Berrang, M.E., Adams, E.S., Meinersmann, R.J. 2016. Triclosan in Campy-Cefex Agar to aid in Enumeration of Naturally Occurring Campylobacter spp. in Broiler Ceca. International Poultry Scientific Forum. January 25-26,2016. Atlanta, GA.

Interpretive Summary:

Technical Abstract: Detecting and enumerating Campylobacter from poultry samples can be difficult without highly selective media because of competing microflora. We have found that adding 0.1 µg/ mL of Triclosan, an antibacterial agent, to Bolton enrichment broth prevents overgrowth of non-Campylobacter bacteria and significantly improves detection in broiler carcass rinse samples. The objective of the current study was to test addition of Triclosan to solid plating media as a means to hold down background microflora during direct plating of cecal samples, making it easier to see and count Campylobacter colonies during enumeration as opposed to enrichment. Campy cefex agar was prepared with and without addition of Triclosan (TCCA and CCA). In eight replications, five or ten sets of fresh ceca were collected from the evisceration line in a commercial broiler processing plant. Ceca (N=75) were smashed to expose contents, diluted 3:1 by weight, mixed, and serial dilutions were direct plated onto TCCA and CCA. All plates were incubated at 42oC for 48 h under micro-aerobic conditions. Characteristic Campylobacter colonies were counted and confirmed as thermophilic Campylobacter by phase contrast microscopy and a latex agglutination test kit. We found that Campylobacter colonies were easier to see and count on TCCA than on CCA. However, there was no difference in the number of Campylobacter counted. A mean of 4.4 log Campylobacter per mL of diluted cecal contents were detected on both TCCA and plain CCA. Although the addition of Triclosan to an enrichment broth is helpful in detection of Campylobacter, adding Triclosan to a solid medium did not improve enumeration of Campylobacter when direct plated.