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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Livestock Bio-Systems » Research » Publications at this Location » Publication #323447
Title: Investigation and validation of the role of a QTL on SSC12 in PCV2b viremia

Author
item TOSKY, EMILY - University Of Nebraska
item Smith, Timothy - Tim
item ENGLE, TAYLOR - University Of Nebraska
item DAVIS, EMILY - University Of Nebraska
item MOURAL, TIMOTHY - University Of Nebraska
item Nonneman, Danny - Dan
item CHIN, JASON - Pacific Biosciences Inc
item BURKEY, THOMAS - University Of Nebraska
item PLASTOW, GRAHAM - University Of Alberta
item KACHMAN, STEPHEN - University Of Nebraska
item CIOBANU, DANIEL - University Of Nebraska

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/11/2016
Publication Date: 1/11/2016
Citation: Tosky, E., Smith, T.P.L., Engle, T., Davis, E., Moural, T., Nonneman, D., Chin, J., Burkey, T., Plastow, G.S., Kachman, S.D., Ciobanu, D.C. 2016. Investigation and validation of the role of a QTL on SSC12 in PCV2b viremia [abstract]. Plant and Animal Genome XXIV Conference Proceedings. Abstract #P0638.

Interpretive Summary:

Technical Abstract: Porcine circovirus type 2 (PCV2) is known to modulate immune response leading to a susceptibility to Porcine Circovirus Associated Diseases (PCVAD) with an impact on growth and mortality. Our previous GWAS of PCV2b experimentally infected pigs uncovered two QTL that explain 14% of the genetic variation of viral load. One of the major SNPs (UNLPCV2.2009, 12.4% VG) was previously unmapped but putatively assigned to the proximal end of SSC12 based on LD analysis. A scaffold of the proximal end of SSC12 was assembled using long-read sequencing. Approximately 600 SNPs, previously unmapped or mapped to SSC12, were assigned to the scaffold, including UNLPCV2.2009. A Bayes-IM association analysis on this scaffold confirmed the major effect of UNLPCV2.2009 on viral load with the CC-genotype being favorable. The effects of this SNP were validated in a PCV2b experimental infection that included all QTL genotypes, with pigs vaccinated for PCV2 as controls (CT and TT genotypes). Among infected animals, viremia of the CC-genotype was lower than TT across time points and CT at 14 dpi (P < 0.05). Compared to the vaccinated, all the infected pigs had higher levels of viremia (P < 0.05) with the exception of the CC-genotype at 7 dpi (P<0.40). The CC-genotype was also associated with lower IgG compared to the other genotypes. No significant differences were observed between genotypes within and between treatment groups for ADG. This study validates previous indication of a relationship between genotype at this locus with reduced PCV2 susceptibility.