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Title: CHARACTERIZATION OF A MURINE MONOCLONAL ANTIBODY GENERATED AGAINST NEOSPORA CANINUM BY WESTERN BLOT ANALYSIS AND IMMUNOELECTRON MICROSCOPY

Author
item COLE R A - AUBURN UNIVERSITY
item LINDSAY D S - AUBURN UNIVERSITY
item DUBEY JITENDER P - 1265-55-00
item TOIVIO-KINNUCAN - AUBURN UNIVERSITY
item BLAGBURN B L - AUBURN UNIVERSITY

Submitted to: American Journal of Veterinary Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/18/1994
Publication Date: N/A
Citation: N/A

Interpretive Summary: Neosporosis is a newly recognized disease caused by the protozoan parasite, Neospora caninum. It can cause abortion and neonatal mortality in livestock and crippling illness in companion animals. Its life cycle and source of infection are unknown. The diagnosis is made at present using a labor-intensive, immunohistochemical test. Scientists at the Beltsville Agricultural Research Center and the Auburn University, Alabama report characteristics of a monoclonal antibody against tachyzoites of Neospora caninum. This monoclonal antibody will be helpful in characterization of antigens for the development of an efficient serologic test for neosporosis.

Technical Abstract: Murine monoclonal antibody 6G7 generated against tachyzoites of Neospora caninum recognized 8 major and several minor antigens as observed in Western blot analysis. Relative rate of migration of the 8 major antigens ranged from 31-97.4 kDa. In addition, Mab 6G7 recognized a T. gondii tachyzoite antigen with a relative rate of migration of 107 kDa. Immunogold labeling of N. caninum tachyzoites grown in human foreskin fibroblast cells indicated that Mab 6G7 binds to micronemes, dense granules, basal portions of rhoptries and intravacuolar tubules within the parasitophorous vacuole. Monoclonal antibody 6G7 also bound to micronemes and basal portions of rhoptries within tachyzoites of Toxoplasma gondii. Monoclonal antibody 6G7 did not significantly inhibit development of tachyzoites in vitro.