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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Cotton Structure and Quality Research » Research » Publications at this Location » Publication #324575
Research Project: Improved Quality Assessments of Cotton from Fiber to Final Products

Location: Cotton Structure and Quality Research

Title: Direct, non-destructive, and rapid evaluation of developmental cotton fibers by ATR FT-IR spectroscopy

Author
item Liu, Yongliang
item Kim, Hee-Jin

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings
Publication Acceptance Date: 1/21/2016
Publication Date: 5/18/2016
Citation: Liu, Y., Kim, H.J. 2016. Direct, non-destructive, and rapid evaluation of developmental cotton fibers by ATR FT-IR spectroscopy. Proceedings of the National Cotton Council Beltwide Cotton Conference,January 5-7, 2016, New Orleans, Louisiana. p. 248-253.

Interpretive Summary: Chemical, compositional, and structural differences within the fibers at different growth stages, as well as their physical properties and end-use qualities, have been investigated considerably through a number of well-defined protocols. Such knowledge is of value to cotton breeders and growers for cotton enhancement and to textile processors for quality control. Due to its direct, non-destructive, and rapid attribute, this study reports the utilization of attenuated total reflection Fourier transform Infrared (ATR FT-IR) spectroscopy method to acquire compositional and structural information of fibers as young as 10 DPA by the strategy of simple algorithmic analysis, and then to compare the secondary cell wall synthesis of fibers at various developing periods.

Technical Abstract: Chemical, compositional, and structural differences within the fibers at different growth stages have been investigated considerably through a number of methodologies. Due to its direct, non-destructive, and rapid attribute, this study reports the utilization of attenuated total reflection Fourier transform Infrared (ATR FT-IR) spectroscopy technique to acquire compositional and structural information of fibers grown in planta and in culture. Principal component analysis (PCA) approach suggested approximately 10-day slower in the transition from primary to secondary cell wall biosyntheses for the fibers grown in culture than for the fibers grown in planta. Relatively, the IR crystallinity index is of interest, as it can detect the slight difference in fibers with day of post anthesis (DPA) larger than 25 days. Furthermore, spectral intensities of two bands at 708 and 730 cm-1 utilized in the ratio algorithm development indicated differing patterns between two types of cotton fibers.