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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #324872
Title: Long non-coding RNA ADNCR suppresses adipogenic differentiation by targeting miR-204

Author
item LI, MINGXUN - Northwest Agricultural & Forestry University
item SUN, XIAOMEI - Northwest Agriculture And Forestry University
item CAI, HANFANG - Northwest Agricultural & Forestry University
item Li, Congjun - Cj
item SUN, YUJIA - Northwest Agricultural & Forestry University
item LAN, CHUZHAO - Northwest Agriculture And Forestry University
item LIN, FENGPENG - Northwest Agriculture And Forestry University
item BAI, YUEYU - Henan Province Institute Of Veterinary Drug Control
item CHEN, HONG - Northwest Agricultural & Forestry University

Submitted to: Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/20/2016
Publication Date: 5/5/2016
Citation: Li, M., Sun, X., Cai, H., Li, C., Sun, Y., Lan, C., Lin, F., Bai, Y., Chen, H. 2016. Long non-coding RNA ADNCR suppresses adipogenic differentiation by targeting miR-204. Genetics. S1874-9399(16)30083-9.

Interpretive Summary: Adipocytes play a vital role in energy homeostasis. The process of cell differentiation by which preadipocytes become adipocytes is regulated by a very complicated network of factors. In this study, a set of adipocyte differentiation-associated long noncoding RNA (ADNCR) was identified and microRNA-204 was identified as the target of ADNCR regulation. Our data not only provide a valuable genomic resource for the identification of lncRNAs with functional roles in adipocyte differentiation but also reveal new insights into understanding the mechanisms of adipogenic differentiation.

Technical Abstract: Adiposeness is a complex and precisely orchestrated process mediated by a network of adipogenic regulatory factors. Several studies have highlighted the relevance of lncRNAs in adipocyte differentiation, but the precise molecular mechanism has largely remained elusive. In the present study, we performed Ribo-Zero RNA-Seq to investigate both the poly(A)+ and poly(A)- lncRNAs of in vitro cultured bovine preadipocytes and differentiated adipocytes. A stringent set of 2,882 lncRNAs was finally identified. A comparison of the lncRNAs expression profiles revealed that 16 lncRNAs are differentially expressed during adipocyte differentiation. We focused on the most downregulated lncRNA, which we named adipocyte differentiation-associated long noncoding RNA (ADNCR). Mechanistically, ADNCR inhibited adipocyte differentiation by functioning as a competing endogenous RNA (ceRNA) for miR-204, thereby augmenting the expression of the miR-204 target gene, SIRT1, which is known to inhibit adipocyte differentiation and adipogenic gene expression by docking with NCoR and SMART to repress PPARgamma activity. Our data not only provide a valuable genomic resource for the identification of lncRNAs with functional roles in adipocyte differentiation but also reveal new insights into understanding the mechanisms of adipogenic differentiation.