Simultaneous determination of aegeline and six coumarins from different parts of the plant Aegle marmelos using UHPLC-PDA-MS and chiral separation of aegeline using HPLC-ToF-MS

Authors: AVULA, BHARATHI - University Of Mississippi; CHITTIBOYINA, AMAR - University Of Mississippi; WANG, YAN-HONG - University Of Mississippi; SAGI, SATYANARAYANARA - University Of Mississippi; RAMAN, VIJAYASANKAR - University Of Mississippi; WANG, MEI - University Of Mississippi; KHAN, IKHLAS - University Of Mississippi

Submitted to: Planta Medica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/4/2016
Publication Date: 4/7/2016
Citation: Avula, B., Chittiboyina, A.G., Wang, Y., Sagi, S.J., Raman, V., Wang, M., Khan, I.A. 2016. Simultaneous determination of aegeline and six coumarins from different parts of the plant Aegle marmelos using UHPLC-PDA-MS and chiral separation of aegeline using HPLC-ToF-MS . Planta Medica. 82(06):580-588.

Interpretive Summary: Aegle marmelos (L.) Corrêa commonly known as bael, or wood apple, belongs to the flowering plant family Rutaceae. It is widely grown in the Indian subcontinent and is found mainly in tropical and subtropical regions. To ensure the identity and quality of A. marmelos raw materials and dietary supplements, a well-documented and validated method was used for the analysis of diverse set of chemicals present in bael. The current study was performed in two parts: in the first part, a simple and rapid method was developed for the determination of seven chemical constituents from A. marmelos and in the second part, an efficient chiral method was developed for the determination of isomers of the compound aegeline. The developed methods were simple and especially suitable for quality control analysis of plant samples and dietary supplements.

Technical Abstract: A fast UHPLC-PDA method was developed for the simultaneous analysis of one alkaloid, aegeline, and six coumarins namely: umbelliferone; scopoletin; marmesinin; 8-hydroxypsoralen angelicin and marmelosin from leaf, fruit, root and bark of Aegle marmelos (L.) Corrêa (Rutaceae). The method was validated for linearity, accuracy, repeatability, limits of detection (LOD) and limits of quantification (LOQ). The linearity range (r2>0.99) of the seven compounds was found to be 0.5-250 µg/mL, LOD and LOQ for seven compounds were found to be 0.1 and 0.5 µg/mL, respectively. The developed UHPLC method is simple, fast, and especially suitable for quality control analysis of coumarins and aegeline from A.marmelos and commercial dietary supplements. Mass spectrometry was used for the identification and confirmation of coumarins and aegeline from different plant parts and dietary supplements. In addition, a novel chiral HPLC-ToF-MS method was developed for enantiomeric separation of aegeline. By applying this method, distribution of enantiomers of aegeline from different parts of A. marmelos and aegeline-containing dietary supplements are reported for the first time.