Identification and taxonomic characterization of Bordetella pseudohinzii spp. nov. isolated from laboratory-raised mice

Authors: IVANOV, YURY - PENNSYLVANIA STATE UNIVERSITY; LINZ, BODO - PENNSYLVANIA STATE UNIVERSITY; REGISTER, KAREN; NEWMAN, JEFFREY - LYCOMING COLLEGE; TAYLOR, DAWN - PENNSYLVANIA STATE UNIVERSITY; BOSCHERT, KENNETH - WASHINGTON UNIVERSITY; LE GUYON, SOAZIG - NANYANG TECHNOLOGICAL UNIVERSITY; WILSON, EMILY - PENNSYLVANIA STATE UNIVERSITY; BRINKAC, LAUREN - J. CRAIG VENTER INSTITUTE; SANKA, RAVI - J. CRAIG VENTER INSTITUTE; GRECO, SUELLEN - WASHINGTON UNIVERSITY; KLENDER, PAULA - WASHINGTON UNIVERSITY; LOSADA, LILIANA - J. CRAIG VENTER INSTITUTE; HARVILL, ERIC - UNIVERSITY OF GEORGIA

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/3/2016
Publication Date: 12/1/2016
Publication URL: https://handle.nal.usda.gov/10113/5695412
Citation: Ivanov, Y., Linz, B., Register, K.B., Newman, J., Taylor, D., Boschert, K., Le Guyon, S., Wilson, E., Brinkac, L., Sanka, R., Greco, S.C., Klender, P.M., Losada, L., Harvill, E. 2016. Identification and taxonomic characterization of Bordetella pseudohinzii spp. nov. isolated from laboratory-raised mice. International Journal of Systematic and Evolutionary Microbiology. 66:5452-5459. doi: 10.1099/ijsem.0.001540.

Interpretive Summary: Bordetella species have historically been subdivided into the "classical” group, represented by the respiratory pathogens B. bronchiseptica, which causes swine atrophic rhinitis and bronchitis and pneumonia in dogs and cats, B. pertussis and B. parapertussis, which cause whooping cough in humans, and six less extensively studied species. The latter "non-classical" group includes B. hinzii, which is known to infect humans, poultry and rabbits. We and others have recently identified from mice a bacterium similar to B. hinzii. Here we report the genome sequence of several such isolates and present data to document our conclusion that Bordetella isolates from mice comprise a novel species, distinct from B. hinzii, for which we propose the name B. pseudohinzii.

Technical Abstract: Bordetella species of the class Betaproteobacteria have historically been subdivided into the "classical” group, represented by the respiratory pathogens B. bronchiseptica, B. pertussis and B. parapertussis, and six less extensively studied species. Among the latter "non-classical" group is B. hinzii, known to infect humans, poultry and rabbits. We and others have recently identified from mice a bacterium similar to B. hinzii. Here, report the genome sequences of several putative B. hinzii isolates from mice and determine their taxonomic relationship to all known Bordetella species based on whole-genome and 16S rRNA phylogenies. We show that B. hinzii isolates are not monophyletic but form two distinct clades. One clade is composed of strains from poultry, humans, and a rabbit, whereas the other is restricted to isolates obtained from laboratory mice. Distinct differences in average nucleotide identity, estimated DNA-DNA hybridization values, gene content, metabolic pathways and host specificity provide evidence for two separate species, B. hinzii (from poultry, humans and rabbit) and a novel species from laboratory mice for which we propose the name B. pseudohinzii.