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ARS Home » Southeast Area » Oxford, Mississippi » Natural Products Utilization Research » Research » Publications at this Location » Publication #325956
Research Project: Discovery and Development of Natural Products for Pharmaceutical and Agrochemical Applications II

Location: Natural Products Utilization Research

Title: Anti-inflammatory activity of constituents isolated from Terminalia chebula ***waiting for publication date

Author
item YANG, MIN HYE - University Of Mississippi
item ALI, ZULFIGAR - University Of Mississippi
item KHAN, IKHLAS - University Of Mississippi
item KHAN, SHABANA - University Of Mississippi

Submitted to: Natural Product Communications
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/12/2014
Publication Date: 7/30/2014
Publication URL: http://handle.nal.usda.gov/10113/62462
Citation: Yang, M., Ali, Z., Khan, I.A., Khan, S.I. 2014. Anti-inflammatory activity of constituents isolated from Terminalia chebula. Natural Product Communications. 9(7):965-968.

Interpretive Summary: Terminalia chebula is a medicinal plant, fruits of which are used in traditional medicine to treat various illnesses. Herbal preparations containing T. chebula as one of the components, have been reported to work against inflammatory conditions. This study was carried out to examine the anti-inflammatory effects of the phytochemicals isolated from the extracts of T. chebula fruits. Four constituents (2 gallotannins and 2 triterpenoids) were found to exhibit anti-inflammatory effects in terms of the inhibition of iNOS and Cox-2 which act as inflammatory process mediators. This was the first report of anti-inflammatory activity of these four constituents mediated through the inhibition of iNOS and Cox-2 in LPS-induced macrophage cell line. The beneficial effect of the plant could be explained partly due to the presence of these constituents.

Technical Abstract: This study was aimed at the evaluation of the anti-inflammatory activity of twelve compounds isolated from the methanolic extract of fruits of Terminalia chebula. The activity was determined in terms of their ability to inhibit inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS stimulated macrophages. Two gallotannins [chebulinic acid (1) and 2,3,6-tri-O-galloyl-B-D-glucose (2)] and two triterpenoids [arjunic acid (3) and arjunolic acid (4)] efficiently reduced nitric oxide (NO) production with IC50 values of 53.4, 55.2, 48.8, and 38.0 µM, respectively. The protein expressions of iNOS and COX-2 were decreased in macrophages by treatment with compounds 1-4 (54-69% and 33-37%, respectively) at 50 µM. This is the first report of anti-inflammatory property of 1-4 mediated by inhibition of iNOS and COX-2 activities at the cellular level.