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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #327542
Title: Quantitative trait loci analysis for net ginning energy requirements in upland cotton (Gossypium hirsutum L.)

Author
item Bechere, Efrem
item Fang, David
item Kebede, Hirut
item Hardin Iv, Robert
item Islam, Md
item Li, Ping
item Scheffler, Jodi

Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/24/2017
Publication Date: 7/4/2017
Citation: Bechere, E., Fang, D.D., Kebede, H.A., Hardin Iv, R.G., Islam, M.S., Li, P., Scheffler, J.A. 2017. Quantitative trait loci analysis for net ginning energy requirements in upland cotton (Gossypium hirsutum L.). Euphytica. 213:160-171. https://doi.org/10.1007/s10681-017-1951-z.
DOI: https://doi.org/10.1007/s10681-017-1951-z

Interpretive Summary: Cotton cultivars that require less force to remove fiber from seed have the potential to be ginned faster with less energy. The objective of this study was to identify quantitative trait loci (QTL) for net ginning energy (NGE) requirement, and its relationship with other fiber quality traits in upland cotton. Cotton cultivar differences in ginning energy requirement are closely related to differences in fiber-seed attachment force which means, the lower the fiber-seed attachment force the lower the ginning energy requirement A mapping population was developed from divergent parents for fiber-seed attachment and net ginning energy (AR 9317-26 with low fiber-seed attachment force and low NGE requirement; TAM 182-34 ELS with higher fiber-seed attachment force and high NGE requirement) to identify genomic regions that influence net ginning energy. Two QTLs located on Chromosomes 12 and 20 were identified. These were closely associated with markers CIR148 and DPL0600. These loci could have played a role in regulating how loose or tight the fibers were attached to the seeds in the cotton lines tested in this study and brought the differences among the lines in net ginning energy requirement. The QTL information on NGE and its association with closely related fiber traits will be valuable in the genetic improvement of overall ginning efficiency.

Technical Abstract: Cotton cultivars with reduced fiber-seed attachment force have the potential to be ginned faster with less energy. The objective of this study was to identify quantitative trait loci (QTL) for net ginning energy (NGE) requirement, and its relationship with other fiber quality traits in upland cotton. Two cotton lines, TAM 182-34 ELS and AR 9317-26, with significant differences in net ginning energy and fiber-seed attachment force, were crossed and 285 F2 plants derived from a single F1 plant were planted in the field and leaf samples collected for DNA extraction. To obtain enough seed cotton to measure net ginning energy requirement, individual F2:3 plants and the two parents were planted in replicated progeny rows. The cotton was ginned on a 10-saw laboratory gin stand. Electrical power used by the gin was measured and recorded with a Yokogawa CW121 power meter. Fiber quality attributes were measured using a High Volume Instrument (HVI). A total of 455 marker loci were used to construct a linkage map. Two QTLs were identified for NGE on chromosomes 12 and 20, associated with markers CIR148 and DPL0600, respectively. The NGE QTLs shared the same genomic region with fuzz percent (FZP) on chromosome 12, supporting the highly significant phenotypic correlation between these two traits. This molecular study reinforces previous suggestions that FZP can be used as an indirect selection tool for NGE. The QTL information on NGE and its association with closely related fiber traits will be valuable in the genetic improvement of overall ginning efficiency.