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ARS Home » Plains Area » Lincoln, Nebraska » Wheat, Sorghum and Forage Research » Research » Publications at this Location » Publication #327738
Title: Wheat streak mosaic virus P1: Defining the minimal region required for the suppression of RNA silencing activity

Author
item GUPTA, ADARSH - University Of Nebraska
item HEIN, GARY - University Of Nebraska
item Tatineni, Satyanarayana - Ts

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2016
Publication Date: 7/15/2016
Citation: Gupta, A., Hein, G.L., Tatineni, S. 2016. Wheat streak mosaic virus P1: Defining the minimal region required for the suppression of RNA silencing activity. American Phytopathological Society Annual Meeting. [abstract] American Phytopathological Society..

Interpretive Summary:

Technical Abstract: Wheat streak mosaic virus (WSMV) is the most economically important wheat virus in the Great Plains region of USA. WSMV is the type species of the genus Tritimovirus in the family Potyviridae, and is transmitted by the wheat curl mite, Aceria tosichella Keifer. Previously, we reported that WSMV P1 functions as the viral suppressor of RNA silencing (VSR) and pathogenicity enhancer. In this study, we examined the minimal region of P1 required for VSR function. Twenty-one deletion mutants of WSMV P1 were generated by progressively deleting from the N- and C-terminal regions. VSR assays were conducted by agro-infiltration of deletion mutants in pCASS4 together with the 35S-GFP into Nicotiana benthamiana leaves. WSMV P1 with deletions comprising up to 23 amino acids at the N-terminal region exhibited VSR activity. While deletion of N-terminal 25 amino acids completely abolished the VSR activity. In contrast, even a single amino acid at the C-terminus is indispensable for VSR activity of P1, suggesting that the C-terminal region of WSMV P1 is crucial for VSR activity. Mechanistic studies of WSMV P1 in VSR activity will be discussed.