Skip to main content
ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Chemistry Research » Research » Publications at this Location » Publication #328329
Title: Jasmonate-mediated stomatal closure under elevated CO2 revealed by time-resolved metabolomics

Author
item GENG, SISI - University Of Florida
item BISWAVAS MISRA, BISWAPRIYA - University Of Florida
item DE ARMAS, EVALDO - Thermo Fisher Scientific
item HUHMAN, DAVID - Samuel Roberts Noble Foundation, Inc
item Alborn, Hans
item SUMNER, LLOYD - Samuel Roberts Noble Foundation, Inc
item CHEN, SIXUE - University Of Florida

Submitted to: Plant Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/1/2016
Publication Date: 12/5/2016
Citation: Geng, S., Biswavas Misra, B., De Armas, E., Huhman, D., Alborn, H.T., Sumner, L.W., Chen, S. 2016. Jasmonate-mediated stomatal closure under elevated CO2 revealed by time-resolved metabolomics. Plant Journal. 88:947-962.

Interpretive Summary: Foliar stomatal movements are critical for regulating plant water status and gas exchange. Elevated carbon dioxide (CO2) concentrations are known to induce stomatal closure. However, current knowledge on CO2 signal transduction in stomatal guard cells is limited. Therefore, a scientist at the chemistry group, USDA, ARS, CMAVE in Gainesville Florida in collaboration with scientist at University of Florida, Samuel Roberts Noble Foundation and Thermo Fisher Scientific utilized several powerful metabolomics techniques for a time course study of 358 metabolites in Brassica napus guard cells during elevated CO2. Major alterations in flavonoid, organic acid, sugar, fatty acid, phenylpropanoid, and amino acid profiles in the guard cells indicated changes in both primary and specialized metabolic pathways and most interestingly, the jasmonic acid signaling pathway appeared to be, at least in part, involved in mediating the stomata closure typically seen in response to elevated CO2. This work adds to our basic understanding of complex metabolic changes in plants in response to environmental changes such as elevated atmospheric CO2 levels.

Technical Abstract: Foliar stomatal movements are critical for regulating plant water status and gas exchange. Elevated carbon dioxide (CO2) concentrations are known to induce stomatal closure. However, current knowledge on CO2 signal transduction in stomatal guard cells is limited. Here we report the metabolomic responses of Brassica napus guard cells to elevated CO2 using three hyphenated metabolomics platforms: gas chromatography (GC)-mass spectrometry (MS), liquid chromatography (LC)-multiple reaction monitoring (MRM)-MS, and ultra high-performance LC (UHPLC)-quadrupole time-of-flight (QToF)-MS. A total of 358 metabolites were quantified in a time-course response of guard cells to elevated CO2 treatment. Most metabolites increased under elevated CO2, and the differences were most significant at 10 minutes. Concomitantly, reactive oxygen species (ROS) production increased and stomatal aperture decreased with time. Major alterations in flavonoid, organic acid, sugar, fatty acid, phenylpropanoid, and amino acid metabolic pathways indicated changes in both primary and specialized metabolic pathways in guard cells. Most interestingly, jasmonic acid (JA) biosynthesis pathway was significantly altered in the course of the elevated CO2 treatment. Together with results obtained from JA biosynthesis and signaling mutants as well as CO2 signaling mutants, we discovered that the CO2 induced stomatal closure is mediated in part by JA signaling.