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Title: Identification of genomic region controlling resistance to aflatoxin contamination in a peanut recombinant inbred line population (Tifrunner x GT-C20)

Author
item AGARWAL, G - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item VISHWAKARMA, M - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item KALE, S - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item NAYAK, S - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item PANDEY, M - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item VARSHNEY, R - INTERNATIONAL CROPS RESEARCH INSTITUTE FOR SEMI-ARID TROPICS (ICRISAT) - INDIA
item JI, X - UNIVERSITY OF GEORGIA
item GUO, X - UNIVERSITY OF GEORGIA
item FOUNTAIN, J - UNIVERSITY OF GEORGIA
item WANG, H - UNIVERSITY OF GEORGIA
item Holbrook, Carl - Corley
item Guo, Baozhu

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2016
Publication Date: 7/12/2016
Citation: Agarwal, G., Vishwakarma, M., Kale, S., Nayak, S.N., Pandey, M., Varshney, R.K., Ji, X., Guo, X., Fountain, J.C., Wang, H., Holbrook Jr, C.C., Guo, B. 2016. Identification of genomic region controlling resistance to aflatoxin contamination in a peanut recombinant inbred line population (Tifrunner x GT-C20) [abstract]. American Peanut Research and Education Society Abstracts.

Interpretive Summary:

Technical Abstract: Aflatoxin contamination of peanut is a significant threat to global food safety. In this study we performed quantitative trait loci (QTL) analysis to identify peanut genomic regions contributing to aflatoxin contamination resistance in a recombinant inbred line (RIL) population derived from the Tifrunner (susceptible) and GT-C20 (resistant). Using an in-vitro kernel screening assay, visible fungal colonization and aflatoxin contents were measured in three repeated tests, each with three technical replicates. Aflatoxin levels and fungal colonization ratings varied among the RILs with a distribution skewed toward lower aflatoxin levels. Using previously determined genotypes for the population, QTL analysis for all replicates was performed and identified a total of 14 QTLs, four QTLs for aflatoxin contamination and ten for fungal colonization. Four major QTLs were identified, each with more than 10% phenotypic variation explained (PVE). One major QTL for aflatoxin contamination had 15.14% PVE and three for fungal colonization had PVEs of 13.23%, 11.21% and 14.17%, respectively. Comparing the major aflatoxin QTL with the peanut diploid reference genome sequences showed that the QTL flanking markers defined a ~99 Mb region containing 1,308 genes on chromosome A04. Genes of interest in this region include LLRs, pathogenesis related genes, NBRs, disease resistance response proteins, MYB transcription factors (TF), and pathogenesis-related thaumatin superfamily proteins. The other three major QTLs identified for fungal colonization were found on chromosomes A03 and A05, spanning ~50.5, 4.4, and 14.8 Mb regions, respectively, each containing genes coding for LRR, NAC, Myb TF, and Zn finger proteins. In order better identify potential candidate genes; more extensive genotyping is currently being performed to increase marker density.