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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #330470
Title: Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

Author
item YASMIN, TUBA - University Of Illinois
item NELSON, BERLIN - North Dakota State University
item HOBBS, HOUSTON - University Of Illinois
item McCoppin, Nancy
item LAMBERT, KRIS - University Of Illinois
item Domier, Leslie

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/3/2016
Publication Date: 12/5/2016
Citation: Yasmin, T., Nelson, B.D., Hobbs, H.A., McCoppin, N.K., Lambert, K.N., Domier, L.L. 2016. Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing. Archives of Virology. 162(4):1089-1092. doi:10.1007/s00705-016-3152-9.

Interpretive Summary: Soybean provides essential nutrients for both humans and food animals, and is an important source of bioenergy. Soybean is a host of diverse microbial pathogens including many viruses that can significantly reduce yields and seed quality. To identify and quantify the viruses infecting soybean in North Dakota, a survey was conducted during the 2010 growing season. Leaf samples were collected from soybean fields in 25 North Dakota counties and analyzed by high-throughput sequencing. The surveys identified a new soybean-infecting virus, Soybean latent spherical virus, that is related to Tobacco ringspot virus. Unlike Tobacco ringspot virus, which can cause severe disease symptoms in soybean plants, soybean plants inoculated with the new virus did not display obvious symptoms. The virus identified in this study adds to the biological diversity of viruses naturally infecting soybean plants. This information will be useful to scientists interested in studying virus-host interactions and synergistic diseases caused by infection of soybean plants by multiple viruses.

Technical Abstract: The complete nucleotide sequence of a new soybean-infecting member of the Nepovirus genus (provisionally named Soybean latent spherical virus [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends, each contained a single long open reading frame and a 3’ noncoding region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in Subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus.