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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #331273
Research Project: Umbrella Project for Food Safety

Location: Warmwater Aquaculture Research Unit

Title: Generation and stabilization of whey-based monodisperse naoemulsions using ultra-high pressure homogenization and small amphipathic co-emulsifier combinations

Author
item ZHANG, XUE - Mississippi State University
item HAQUE, ZAHUR - Mississippi State University

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/20/2015
Publication Date: 10/20/2015
Citation: Zhang, X., Haque, Z.Z. 2015. Generation and stabilization of whey-based monodisperse naoemulsions using ultra-high pressure homogenization and small amphipathic co-emulsifier combinations. Journal of Agricultural and Food Chemistry. 63(45):10070-10077.

Interpretive Summary: The study aimed to study the use of ultra-high pressure homogenization to produce and evaluate stable monodispered nanoemulsions in a narrow size distribution within a desired nanosize range of <100 nm, a size range defined as the desired nanoglobular range (DNR), using WPC as the primary emulsifier, with and without one or more co-emulsifiers consisting of anionic sodium dodecyl sulfate.

Technical Abstract: Ultra-high-pressure homogenization (UHPH) was used to generate monodisperse stable peanut oil nanoemulsions within a desired nanosize range (<100 nm) (DNR) stabilized using combinations of whey protein concentrate (WPC), sodium dodecyl sulfate, Triton X-100 (X100), and zwitterionic sulfobetaine-based surfactants differing in hydrophobicity. For WPC [2.0% (w/v)], the dispersed-phase fractions (f) of 0.05 and 210 MPa significantly reduced the mean globule size (dvs) but the grouped frequency distribution was bimodal and larger than that of DNR. Addition of coemulsifier sulfobetaine 3-10 (SB3-10) [7.5% (w/w) WPC] gave particles within DNR (dvs of 73 nm) though still in a bimodal distribution. Circular dichroism prior to UHPH showed little disruption of the secondary structure of proteins in WPC by SB3-10, whereas X100 obliterated it. A WPC/SB3-10 mixture retained some periodic structure even when mixed with X100 [10% (w/w) WPC] and remarkably gave a narrow monomodal distribution within DNR with the highest stability reflected by a lack of creaming after storage for 30 days (22 °C).