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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #331292
Title: Fine mapping of a Phytophthora-resistance gene RpsWY in soybean (Glycine max L.) by high-throughput genome-wide sequencing

Author
item CHENG, YANBO - South China Agricultural University
item MA, QIBIN - South China Agricultural University
item REN, HAILONG - South China Agricultural University
item XIA, QIUJU - Bgi Shenzhen
item SONG, ENLIANG - South China Agricultural University
item TAN, ZHIYUAN - South China Agricultural University
item Li, Shuxian
item ZHANG, GENGYUN - Bgi Shenzhen
item NIAN, HAI - South China Agricultural University

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/26/2017
Publication Date: 5/1/2017
Citation: Cheng, Y., Ma, Q., Ren, H., Xia, Q., Song, E., Tan, Z., Li, S., Zhang, G., Nian, H. 2017. Fine mapping of a Phytophthora-resistance gene RpsWY in soybean (Glycine max L.) by high-throughput genome-wide sequencing. Theoretical and Applied Genetics. 130(5):1041-1051. https://doi.org/10.1007/S00122-017-2869-5.
DOI: https://doi.org/10.1007/S00122-017-2869-5

Interpretive Summary: Phytophthora root rot (PRR) is one of the most important soil-borne diseases in many soybean-production regions in the world. Identification of resistant gene(s) is an effective way for breeding to prevent soybean from being harmed by this disease. In this study, two soybean populations developed from crossing a susceptible cultivar Huachun 2 with the resistant cultivar Wayao, were developed to map the resistance (Rps) gene. Our results indicated that PRR resistance in Wayao was controlled by a single dominant gene, temporarily named RpsWY, which was mapped on chromosome 3. The RpsWY gene was located in the molecular marker bin 401 between 4466230 and 4502773 bp on chromosome 3. Four predicted genes also were identified in this region. These results suggest that the high-throughput genome-wide resequencing is an effective method to finely map PRR candidate genes.

Technical Abstract: Phytophthora root rot (PRR) caused by Phytophthora sojae is one of the most important soil-borne diseases in many soybean-production regions in the world. Identification of resistant gene(s) is an effective way for breeding to prevent soybean from being harmed by this disease. Here, two soybean populations of 191 F2 individuals and 196 F7:8 recombinant inbred lines (RILs) were developed to map Rps gene by crossing a susceptible cultivar Huachun 2 with the resistant cultivar Wayao, respectively. Heredity analysis indicated that PRR resistance in Wayao was controlled by a single dominant gene, temporarily named RpsWY, which was mapped on chromosome 3 using F2 population. A high-density genetic linkage bin map was constructed using 3469 recombination bins of the RILs to explore the candidate genes by the high-throughput genome-wide sequencing. The results of genotype analysis showed that the RpsWY gene was located in bin 401 between 4466230 and 4502773 bp on chromosome 3 through RIL 71 and 100. Four predicted genes (Glyma03g04350, Glyma03g04360, Glyma03g04370 and Glyma03g04380) were found at the narrowed region of 36.5 kb in bin 401. These results suggest that the high-throughput genome-wide resequencing is an effective method to finely map PRR candidate genes.