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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #331405
Title: Molecular markers developed for Pouteria sapota

Author
item MARTINEZ-CASTILLO, JAIME - Centro De Investigacion
item Arias De Ares, Renee

Submitted to: Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/22/2016
Publication Date: N/A
Citation: N/A

Interpretive Summary: none required. abstract only. dr

Technical Abstract: The tropical plant Pouteria sapota (Jacq.) is known for its edible fruits that contain unique carotenoids, and for the chemicals extracted from its bark, leaves and roots having fungitoxic, insecticidal, anti-inflamatory, anti-oxidant and tyrosinase inhibitory activities. We reported high throughput sequencing of microsatellite-enriched libraries of P. sapota, submitted 5223 DNA contig sequences to NCBI GenBank, developed 384 microsatellites markers and tested the latter on 31 P. sapota samples from 11 populations from Mexico. BLAST analysis to gene ontology of the DNA sequences containing microsatellites that effectively discriminated these populations, showed association to physiological activities. In samples collected from the wild, microsatellites at 62 loci detected one or more alleles that were not observed among the cultivated populations tested. Potential applications of the developed microsatellite markers screening germplasm collections, for ancestry and domestication studies are discussed.