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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Bioenergy Research » Research » Publications at this Location » Publication #332191
Title: Rapid identification of Robinsoniella peoriensis using specific 16S rRNA gene PCR primers

Author
item Whitehead, Terence
item ANOMA, CHRISTELLE - Gateway Technical College
item MCLAUGHLIN, RICHARD - Gateway Technical College

Submitted to: Anaerobe
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/23/2016
Publication Date: 11/24/2016
Publication URL: https://handle.nal.usda.gov/10113/5628628
Citation: Whitehead, T.R., Anoma, C., McLaughlin, R.W. 2016. Rapid identification of Robinsoniella peoriensis using specific 16S rRNA gene PCR primers. Anaerobe. 43:39-42. doi: 10.1016/j.anaerobe.2016.11.008.

Interpretive Summary: Antimicrobial compounds have been commonly used as feed additives for domestic animals to reduce infection and promote growth. Recent reports have suggested such feeding practices may result in increased microbial resistance to antibiotics, which can have an impact on human health. The bacterium Robinsoniella peoriensis was previously isolated and characterized in our laboratory from swine feces and stored manure. Strains of this bacterium have recently also been isolated from human infections and other ecosystems. Such strains were collected by us from around the world and found to be resistant to a number of antibiotics. Rapid identification of this microorganism would be of great help to medical and other laboratories. In the current study, we report on the development of polymerase chain reaction primer sets to identify this bacterium. Such a method will be of great help to medical laboratories and other scientists to rapidly and accurately identify Robinsoniella peoriensis.

Technical Abstract: Robinsoniella peoriensis is a Gram-positive, spore-forming anaerobic bacterium initially isolated and characterized from swine manure and feces. Since then strains of this species have been identified from a variety of mammalian and other gastrointestinal tracts. More recently strains of this species have been isolated from a plethora of human infections. Therefore, it is of great interest to develop methods to rapidly identify this microorganism in the medical and other laboratories. This report describes the use of PCR primers targeting the 16S rRNA gene of Robinsoniella peoriensis to identify strains of this bacterium.