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Title: Genetic characterization of glossy-leafed mutant broccoli lines

Author
item Branham, Sandra
item Farnham, Mark

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/28/2016
Publication Date: 1/15/2017
Citation: Branham, S., Farnham, M.W. 2017. Genetic characterization of glossy-leafed mutant broccoli lines. Plant and Animal Genome Conference. P0456.

Interpretive Summary: N/A

Technical Abstract: Glossy mutants of Brassica oleracea L. have reduced or altered epicuticular wax on the surface of their leaves as compared to wild-type plants, conveying a shiny green appearance. Mutations conferring glossiness are common and have been found in most B. oleracea crop varieties, including cauliflower, kale, broccoli, Brussels sprouts, cabbage, and collard. Glossiness can decrease pest infestation and damage through reduced herbivore oviposition and feeding and increased efficiency of predatory wasps. The genetic mutations that cause glossiness have not been identified in B. oleracea. We used genotyping-by-sequencing (GBS) to search for polymorphisms between two pairs of near-isogenic lines (NILs) that differ only for glossiness. The results suggest that the two glossy mutant lines were likely caused by mutations to genes encoding enzymes essential to different steps of the wax synthesis pathway.