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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #336301
Title: Identification of genetic determinants in potato for resistance to Verticillium wilt

Author
item KUMAR, ARUN - University Of Wisconsin
item Jansky, Shelley
item Halterman, Dennis

Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2016
Publication Date: 1/13/2017
Citation: Kumar, A., Jansky, S.H., Halterman, D.A. 2017. Identification of genetic determinants in potato for resistance to Verticillium wilt [abstract]. Plant and Animal Genome. Paper No. P0816.

Interpretive Summary:

Technical Abstract: Despite decades of research to control Verticillium wilt (VW), which is caused by the soil-borne fungal pathogens Verticillium dahliae and V. albo-atrum, this disease continues to be a recurrent problem for potato production throughout North America. It can result in yield losses of up to 50% and is currently controlled by fumigation. In tomato, resistance to VW is governed by resistance gene Ve, which has been exploited in breeding programs from more than 50 years. However, in potato, our studies indicate that even though Ve is a major dominant resistance gene, it is not sufficient for complete resistance. Two homozygous diploid parents, S. tuberosum DM1-3 (susceptible to VW) and S. chacoense M6 (resistant to VW) were crossed and an F2 population was created by selfing of one F1 individual. Since M6 is homozygous for the dominant Ve gene, we were expecting three quarters of the F2 population to be resistant. However, in field and greenhouse experiments, more than 70% of the F2 clones were susceptible. This suggests that there are other signaling components necessary for proper function of Ve and they are segregating in the F2 population. Marker analysis of the Ve locus was done using diagnostic primer pairs, but analysis was complicated by gene duplications in the region. Additionally, we have SNP genotyped the population and will map other major or minor regions in the genome responsible for VW resistance. The outcomes of study will help with the development of cultivars with higher levels of resistance to VW.