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Title: Genome sequence variation in the constricta strain dramatically alters the protein interaction and localization map of Potato yellow dwarf virus

Author
item JANG, CHANYONG - University Of Kentucky
item WANG, RENYUAN - University Of Kentucky
item WELLS, JOSEPH - University Of Kentucky
item LEON, FABIAN - University Of Kentucky
item FARMAN, MARK - University Of Kentucky
item Hammond, John
item GOODIN, MICHAEL - University Of Kentucky

Submitted to: Journal of General Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/10/2017
Publication Date: 6/21/2017
Citation: Jang, C., Wang, R., Wells, J., Leon, F., Farman, M., Hammond, J., Goodin, M. 2017. Genome sequence variation in the constricta strain dramatically alters the protein interaction and localization map of Potato yellow dwarf virus. Journal of General Virology. 98(6):1526-1536. https://doi.org/10.1099/jgv.0.000771.
DOI: https://doi.org/10.1099/jgv.0.000771

Interpretive Summary: The interactions between plant viruses, plant hosts, and their vectors, determine host and vector specificity and relative severity of disease symptoms. Potato yellow dwarf virus, a highly destructive pathogen of potato with two distinct strains, has been studied extensively; however, information about these biologically distinct strains was not available. A team of scientists identified multiple differences in interactions between the viral proteins of these strains, which differ in insect vector specificity and disease severity. This information will be valuable to other scientists interested in virus/host/vector interactions and could lead to identification of host interactions that can become the target for plant breeders to develop virus resistance, with the long term goal of minimizing virus infection and disease damage affecting crop productivity.

Technical Abstract: The genome sequence of the constricta strain of Potato yellow dwarf virus (CYDV) was determined to be 12,792 nucleotides long and organized into seven open reading frames with the gene order 3’-N-X-P-Y-M-G-L-5’, which encodes the nucleocapsid, phosphoprotein, movement, matrix, glycoprotein and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. Cloned open reading frames for each gene, except L, were used to construct a protein interaction and localization map (PILM) for this virus, which shares greater than 80% sequence similarity in all ORFs except X and P with the sanguinolenta strain of this species (SYDV). Protein localization patterns and interactions unique to each viral strain were identified, resulting in strain-specific PILMs. Mapping of CYDV and SYDV proteins in the context of virus infected cells pointed to subcellular loci likely to be sites of replication, morphogenesis and movement.