Proteomic analysis of lung tissue by DIGE

Authors: Nally, Jarlath; SCHULLER, SIMONE - University Of Bern

Submitted to: Methods in Molecular Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/10/2017
Publication Date: 10/11/2017
Citation: Nally, J.E., Schuller, S. 2017. Proteomic analysis of lung tissue by DIGE. Methods in Molecular Biology. 1664:167-183. https://doi:10.1007/978-1-4939-7268-5_14.
DOI: https://doi.org/10.1007/978-1-4939-7268-5_14

Interpretive Summary: The human lung performs an essential physiological function via complex regulatory pathways. Diseases of the human lung are modeled in guinea pigs. In order to understand how the protein composition of lungs change in response to specific disease processes, we describe the application of 2-dimensional Difference Gel Electrophoresis (DIGE) as a means to characterize and quantify differential protein expression in diseased lungs of guinea pigs compared to normal non-diseased controls.

Technical Abstract: Lungs perform an essential physiological function, mediated by a complex series of events that involve the coordination of multiple cell types to support not only gaseous exchange, but homeostasis and protection from infection. Guinea pigs are an important animal disease model for a number of infectious and non-infectious pulmonary conditions and the availability of a complete genome facilitates comprehensive analysis of tissues using the tools of proteomics. Here we describe the application of 2-D Difference Gel Electrophoresis (DIGE) to compare, quantify and identify differential protein expression of proteins in lung tissue from guinea pigs with leptospiral pulmonary hmorrhage syndrome (LPHS) compared to non-infected controls. 2-D DIGE is a powerful technique that provides novel insights into the dynamics of the complex lung proteome during health and disease.