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Title: Fine Mapping identifies CsGCN5 encoding a histone acetyltransferase as putative candidate gene for tendril-less1 mutation (td-1) in cucumber

Author
item CHEN, FEIFAN - Northwest Agricultural & Forestry University
item FU, BINGBING - Northwest Agricultural & Forestry University
item PAN, YUPENG - University Of Wisconsin
item WEN, HAIFAN - Northwest Agricultural & Forestry University
item CHEN, PENG - Northwest Agricultural & Forestry University
item Weng, Yiqun
item LI, YUHONG - Northwest Agricultural & Forestry University

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/15/2017
Publication Date: 5/2/2017
Publication URL: http://handle.nal.usda.gov/10113/5746617
Citation: Chen, F., Fu, B., Pan, Y., Zhang, C., Wen, H., Weng, Y., Chen, P., Li, Y. 2017. Fine mapping identifies CsGCN5 encoding a histone acetyltransferase as putative candidate gene for tendril-less1 mutation (td-1) in cucumber. Theoretical and Applied Genetics. 130(8):1549-1558. doi: 10.1007/s00122-017-2909-1.

Interpretive Summary: Tendril initiated from the lateral meristem is an important and characteristic organ for the species in the Cucurbitaceae family including cucumber (Cucumis sativus L.). While tendril has its evolutionary significance, it also poses a nuisance in cucumber cultivation in protected environments in which tendril-less cucumber has its advantages. From an EMS mutagenesis population, we identified a tendril-less mutant B007, which was shown to be controlled by a recessive gene td-1. Through next-generation sequencing-aided map-based cloning, we show that CsGCN5, a cucumber gene for a histone acetyltransferase as the most possible candidate gene for td-1. A non-synonymous SNP in on the first exon of CsGCN5 was responsible for the tendril-less mutation, which resulted in an amino acid substitution from Asp (D) in the wild type to Asn (N) in the mutant. The candidacy was further confirmed by multiple lines of evidence in both biparental and natural cucumber populations. Quantitative gene expression revealed non-significant differential expression of CsGCN5 in multiple organs. CsGCN5 exhibited strong expression in the tendril of wild-type plants suggesting its important roles in growth and development of plant tendril. Identification and characterization of the td-1 mutant from the present study provided a useful tool in understanding the molecular mechanisms of tendril organogenesis and investigation of novel functions of the histone acetyltransferase in cucumber.

Technical Abstract: The tendril is originated from the lateral meristem, and it is an important and characteristic organ for the species in the Cucurbitaceae family including cucumber. While tendril has its evolutionary significance, it also poses a nuisance in cucumber cultivation in protected environments in which tendril-less cucumber has its advantages. From an EMS mutagenesis population, we identified a tendril-less mutant B007. We provided evidence that this mutation is controlled by a recessive gene td-1. Through next-generation sequencing-aided map-based cloning, we show that CsGCN5, a cucumber gene for a histone acetyltransferase as the most possible candidate gene for td-1. A non-synonymous SNP in on the first exon of CsGCN5 was responsible for the tendril-less mutation, which resulted in an amino acid substitution and possible alternation of protein functions in the mutant. The candidacy was further confirmed by multiple lines of evidence in both biparental and natural cucumber populations. Quantitative gene expression revealed non-significant differential expression of CsGCN5 in multiple organs. CsGCN5 exhibited strong expression in the tendril of wild-type plants suggesting its important roles in growth and development of plant tendril. Identification and characterization of the td-1 mutant from the present study provided a useful tool in understanding the molecular mechanisms of tendril organogenesis and investigation of novel functions of the histone acetyltransferase in cucumber.