Characterization and use of new monoclonal antibodies to CD11c,CD14, and CD163 to analyze the phenotypic complexity of ruminantmonocyte subsets

Authors: ELNAGGAR, MAHMOUD - Washington State University; ABDELLRAZEQ, GRABER - Washington State University; MACK, VICTORIA - Washington State University; Fry, Lindsay; DAVIS, WILLIAM - Washington State University; PARK, TAEK KUN - Seoul National University

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/25/2016
Publication Date: 6/7/2016
Citation: Elnaggar, M.M., Abdellrazeq, G.S., Mack, V., Fry, L.M., Davis, W.C., Park, T. 2016. Characterization and use of new monoclonal antibodies to CD11c,CD14, and CD163 to analyze the phenotypic complexity of ruminantmonocyte subsets. Veterinary Immunology and Immunopathology. 178:57-63.

Interpretive Summary: New markers to identify immune response cells in cattle and sheep were identified and characterized. This work provides reagents to be used in characterization of immune responses in natural disease and for vaccine development. These particular reagents identify monocyte populations.

Technical Abstract: The sequencing of the bovine genome and development of mass spectrometry, in conjunction with flow cytometry (FC), have afforded an opportunity to complete the characterization of the specificity of monoclonal antibodies (mAbs), only partially characterized during previous international workshops focused on antibody development for livestock (1991, Leukocyte Antigens in Cattle, Sheep, and Goats; 1993, Leukocyte Antigens of Cattle and Sheep; 1996, Third Workshop on Ruminant Leukocyte Antigens). Theobjective of this study was to complete the characterization of twelve mAbs incompletely characterized during the workshops that reacted with molecules predominantly expressed on bovine monocytes and use them to provide further information on the phenotypic complexity of monocyte subsets in ruminants. Analysis revealed that the mAbs could be grouped into three clusters that recognize three different molecules: CD11c, CD14, and CD163. Following characterization, comparison of the patterns of expression of CD14 and CD163 with expression of CD16, CD172a, and CD209 revealed the mononuclear cell population is comprised of multiple subsets with differential expression of these molecules. Further analysis revealed the epitopes recognized by mAbs to CD14 and CD163 are conserved on orthologues in sheep and goats. In contrast to CD14 that is also expressed on sheep and goat granulocytes, CD163 is a definitive marker for their monocytes. ©