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ARS Home » Northeast Area » Orono, Maine » National Cold Water Marine Aquaculture Center » Research » Publications at this Location » Publication #339582
Title: Innate immune response of channel catfish (Ictalurus punctatus) mannose-binding lectin to channel catfish virus

Author
item OURTH, D - University Of Memphis
item RAGHU, D - University Of Memphis
item MARECAUX, E - University Of Memphis
item Peterson, Brian

Submitted to: Diseases of Aquatic Organisms
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/15/2017
Publication Date: 6/15/2017
Citation: Ourth, D.D., Raghu, D., Marecaux, E., Peterson, B.C. 2017. Innate immune response of channel catfish (Ictalurus punctatus) mannose-binding lectin to channel catfish virus. Diseases of Aquatic Organisms. 124:159-163.

Interpretive Summary: The channel catfish virus (CCV) is a pathogenic herpesvirus that infects channel catfish in pond aquaculture in the Southeast USA. Mannose-binding lectin (MBL) could play an important role in the innate response of channel catfish by binding to CCV. Cell cultures of CCV were grown in channel catfish ovary cells (CCOC). A dot-immunoblot enzyme-linked immunosorbent assay was done to determine the binding ability of 5-months old channel catfish serum to CCOC infected with the CCV. Two separate nitrocellulose membrane blotting techniques were done using uninfected and infected CCOC. The uninfected CCOC decreased by 29.3% and 33.4% in their binding of channel catfish MBL when compared with infected CCOC. The average binding ability of channel catfish MBL towards infected CCOC was therefore 31.4% greater when comparing the infected and uninfected CCOC. This demonstrates that the binding of catfish serum MBL to CCV is concentration dependent and related to serum concentrations of MBL. MBL may play an important role in binding to CCV and reducing mortality in catfish infected with this devastating disease.

Technical Abstract: The channel catfish virus (CCV) is a pathogenic herpesvirus that infects channel catfish (Ictalurus punctatus) in pond aquaculture in the Southeast USA. The innate immune protein mannose-binding lectin (MBL) could play an important role in the innate response of channel catfish by binding to the CCV. Cell cultures of the CCV were grown in channel catfish ovary cells (CCOC). A dot-immunoblot enzyme-linked immunosorbent assay was done to determine the binding ability of 5-months old channel catfish serum MBL (26.2 µg/ml MBL) to CCOC infected with the CCV. Two separate nitrocellulose membrane blotting techniques were done using uninfected and infected CCOC. The uninfected CCOC decreased by 29.3% and 33.4% in their binding of channel catfish MBL when compared with infected CCOC using the two membrane procedures. The average binding ability of channel catfish MBL towards infected CCOC was therefore 31.4% greater when comparing the infected and uninfected CCOC. Normalization equation values of MBL for the 5-months catfish were compared for the two membrane binding procedures. The two normalization values were very close (142 and 150) in binding ability of MBL to the infected CCOC. The 5-months catfish serum had twice the concentration of MBL (26.2 µg/ml MBL) when compared with the 7-months catfish serum (13.2 µg/ml MBL) and was 2.4 times greater when comparing the infected and uninfected catfish binding percentages. This demonstrates that the binding of channel catfish serum MBL to the CCV is concentration dependent and related to serum concentrations of MBL.