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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #340220
Title: Development and validation of SNP markers for QTL underlying resistance to stripe rust in common wheat P10057

Author
item WU, JIANHUI - Northwest Agriculture And Forestry University
item WANG, QILIN - Northwest Agriculture And Forestry University
item Chen, Xianming
item LIU, SHENGJIE - Northwest Agriculture And Forestry University
item LI, HAIYAN - Northwest Agriculture And Forestry University
item ZEN, QINGDONG - Northwest Agriculture And Forestry University
item MU, JINGMEI - Northwest Agriculture And Forestry University
item DAI, MIAOFEI - Northwest Agriculture And Forestry University
item HAN, DEJUN - Northwest Agriculture And Forestry University
item KANG, ZHENSHENG - Northwest Agriculture And Forestry University

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/15/2017
Publication Date: 12/20/2017
Citation: Wu, J., Wang, Q., Chen, X., Liu, S., Li, H., Zen, Q., Mu, J., Dai, M., Han, D., Kang, Z. 2017. Development and validation of SNP markers for QTL underlying resistance to stripe rust in common wheat P10057. Plant Disease. 101(12):2079-2087.

Interpretive Summary: Stripe rust is an important disease of wheat worldwide. Utilization of adult plant resistance (APR) constitutes a strategy to sustainably control this disease. Wheat cultivar P10057 exhibits a high level of APR to stripe rust in field environments. The goal of this study was to identify quantitative trait loci (QTL) involved in stripe rust resistance in P10057. Three wheat populations were used, namely, 154 F2:6 recombinant inbred lines (RILs) derived from the cross Mingxian 169 × P10057, and 161 and 140 F2:3 lines from Avocet S × P10057 and Zhengmai 9023 × P10057, respectively. These populations were evaluated for infection type (IT) and disease severity (DS) in two field locations in the 2014-2015 and 2015-2016 cropping seasons. Genotyping was done using molecular markers linked to the resistance loci. QTL analysis detected two genomic regions on chromosomes 2BS and 3BS associated with resistance. These two stable QTL, designated QYrlov.nwafu-2BS and Qyrlov.nwafu-3BS, were detected across all environments and explained 22.6–24.6% and 20.6–43.2% of phenotypic variance, respectively. Markers 90K-2BMP19 and 90K-2BMP35 linked QYrlov.nwafu-2BS and 90K-3BSMP4 and 90K-3BSMP7 linked to Qyrlov.nwafu-3BS were reliable for marker-assisted selection in the Zhengmai 9023 × P10057 population. These QTL with KASP markers are potentially useful for improving stripe rust resistance in developing wheat cultivars.

Technical Abstract: Stripe rust is an important disease of wheat worldwide. Utilization of adult plant resistance (APR) constitutes a strategy to sustainably control this disease. Wheat cultivar P10057 exhibits a high level of APR to stripe rust in field environments. The goal of this study was to identify quantitative trait loci (QTL) involved in stripe rust resistance in P10057. Three wheat populations were used, namely, 154 F2:6 recombinant inbred lines (RILs) derived from the cross Mingxian 169 × P10057, and 161 and 140 F2:3 lines from Avocet S × P10057 and Zhengmai 9023 × P10057, respectively. These populations were evaluated for infection type (IT) and disease severity (DS) in two field locations in the 2014-2015 and 2015-2016 cropping seasons. Genotyping was done using simple sequence repeat (SSR) and Kompetitive Allelic Specific (KASP) PCR markers linked to the resistance loci. QTL analysis detected two genomic regions on chromosomes 2BS and 3BS associated with resistance. These two stable QTL, designated QYrlov.nwafu-2BS and Qyrlov.nwafu-3BS,were detected across all environments and explained 22.6–24.6% and 20.6–43.2% of phenotypic variance, respectively. QYrlov.nwafu-2BS may be the resistance allele derived from CIMMYT germplasm and Qyrlov.nwafu-3BS likely corresponds to the locus Sr2/Lr27/Yr30/Pbc. The KASP markers 90K-2BMP19 and 90K-2BMP35 linked QYrlov.nwafu-2BS and 90K-3BSMP4 and 90K-3BSMP7 linked to Qyrlov.nwafu-3BS were reliable for marker-assisted selection (MAS) in the Zhengmai 9023 × P10057 population. These QTL with KASP markers are potentially useful for improving stripe rust resistance in developing wheat cultivars.