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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Animal Health Genomics » Research » Publications at this Location » Publication #340534
Research Project: Genomic Intervention Strategies to Prevent and/or Treat Respiratory Diseases of Ruminants

Location: Animal Health Genomics

Title: Phenotyping and susceptibility of established porcine cells lines to African swine fever virus infection and viral production

Author
item SANCHEZ, ELENA - University Of Madrid
item RIERA, ELENA - University Of Madrid
item NOGAL, MARISA - University Of Madrid
item GALLARDO, CARMINA - Instituto Nacional De Tecnologia Agropecuaria
item FERNANDEZ, PALOMA - University Of Madrid
item BELLO-MORALES, RAQUEL - University Of Madrid
item LOPEZ-GUERRERO, JOSE ANTONIO - University Of Madrid
item Chitko-Mckown, Carol
item RICHT, JURGEN - Kansas State University
item REVILLA, YOLANDA - University Of Madrid

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/1/2017
Publication Date: 9/4/2017
Citation: Sanchez, E.G., Riera, E., Nogal, M., Gallardo, C., Fernandez, P., Bello-Morales, R., Lopez-Guerrero, J., Chitko-McKown, C.G., Richt, J.A., Revilla, Y. 2017. Phenotyping and susceptibility of established porcine cells lines to African swine fever virus infection and viral production. Scientific Reports. 7:10369. https://doi.org/10.1038/s41598-017-09948-x.

Interpretive Summary: African swine fever virus (ASFV) is a highly pathogenic virus that infects cells of monocyte/macrophage lineage – these are phagocytic white blood cells with a single nucleus. This virus effects pigs and causes major economic and health losses worldwide, but is not present in the United States. In this study, four established pig cell lines were compared to pig lung macrophages in terms of cell surface markers, susceptibility to ASFV infection, and virus production. Two virulent strains of ASFV as well as a less pathogenic strain were used in the study. All of the cell lines tested had only low levels of macrophage cell surface receptors, and were only lowly infected by ASFV. Only one cell line which was developed from pig lungs showed more efficient production of the attenuated ASFV but not of the virulent strains. Macrophages are not present in very high numbers in pig lungs, and are difficult and time-consuming to collect. If a cell line could be identified that is efficiently infected by and can produce ASFV, research on this important pig disease could advance much more rapidly and would decrease the number of live pigs needed for experiments.

Technical Abstract: African swine fever virus (ASFV) is a highly pathogenic, double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage, affecting swine species and provoking severe economic losses and health threats. In the present study, four established porcine cell lines, IPAM-WT, IPAM-CD163, C'2+ and WSL, were compared to porcine alveolar macrophage (PAM) in terms of surface marker phenotype, susceptibility to ASFV infection and virus production. The virulent ASFV Armenia/07, E70 or the naturally attenuated NHV/P68 strains were used as viral models. Cells expressed only low levels of specific receptors linked to the monocyte/macrophage lineage, with low levels of infection overall, with the exception of WSL, which showed more efficient production of strain NHV/P68 but not of strains E70 and Armenia/07.