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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Cotton Fiber Bioscience Research » Research » Publications at this Location » Publication #340616
Title: Genome-wide analysis of gene expression of EMS-induced short fiber mutant Ligon lintless-y (liy) in cotton (Gossypium hirsutum L.)

Author
item Naoumkina, Marina
item Bechere, Efrem
item Fang, David
item Thyssen, Gregory
item Florane, Christopher

Submitted to: Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/30/2017
Publication Date: 6/25/2017
Citation: Naoumkina, M.A., Bechere, E., Fang, D.D., Thyssen, G.N., Florane, C.B. 2017. Genome-wide analysis of gene expression of EMS-induced short fiber mutant Ligon lintless-y (liy) in cotton (Gossypium hirsutum L.). Genomics. 109:320-329.

Interpretive Summary: The length of cotton fiber is an important agronomic trait that directly affects the quality of yarn and fabric. Cotton fiber mutants have been useful tools to study the molecular processes of fiber development. In this work we describe a chemically-induced short fiber mutant Ligon-lintless-y (liy), which is controlled by a single recessive locus and affected multiple traits, including height of the plant, and length and maturity of fiber. In this study we explored transcriptional changes in developing fibers of liy mutant line. Our results revealed that many metabolic pathways and other processes were substantially altered in liy developing fibers. Particularly, we discuss perturbed expression of genes involved in signal transduction and biosynthesis of phytohormones, which regulate growth and development. The results of this study provide new insights into transcriptional regulation of cotton fiber development.

Technical Abstract: The length of cotton fiber is an important agronomic trait characteristic that directly affects the quality of yarn and fabric. Cotton fiber mutants have been useful tools to study the molecular processes of fiber development. In this work we describe a chemically-induced short fiber mutant Ligon-lintless-y (liy), which is controlled by a single recessive locus and affected multiple traits, including height of the plant, and length and maturity of fiber. An RNAseq analysis was used to evaluate global transcriptional changes during cotton fiber development at 3, 8 and 16 days post anthesis triggered by liy mutation. We found a large number of significantly differentially expressed genes between liy mutant and its wild type with very little overlap between tested time points. Gene set enrichment analysis revealed that many metabolic pathways and other processes were substantially altered in liy developing fibers. Particularly, we discuss perturbed expression of genes involved in signal transduction and biosynthesis of phytohormones, such as auxin, abscisic acid, gibberellin and ethylene. These results suggest that transcriptional changes in multiple metabolic pathways are a result of altered regulation of phytohormones signal pathways and transcription factors in liy mutant. The results of this study provide new insights into transcriptional regulation of cotton fiber development.