Author
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SHI, LINGLING - JINAN UNIVERSITY |
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GUO, YUNFEI - UNIVERSITY OF SOUTHERN CALIFORNIA |
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DONG, CHENGLIANG - UNIVERSITY OF SOUTHERN CALIFORNIA |
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HUDDLESTON, JOHN - UNIVERSITY OF WASHINGTON |
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YANG, HUI - UNIVERSITY OF SOUTHERN CALIFORNIA |
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HAN, XIAOLU - UNIVERSITY OF SOUTHERN CALIFORNIA |
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FU, AISI - WUHAN UNIVERSITY |
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LI, QUAN - UNIVERSITY OF SOUTHERN CALIFORNIA |
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LI, NA - JINAN UNIVERSITY |
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GONG, SIYI - JINAN UNIVERSITY |
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LINTNER, KATHERINE - THE OHIO STATE UNIVERSITY |
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DING, QIONG - WUHAN UNIVERSITY |
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WANG, ZOU - WUHAN UNIVERSITY |
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HU, JIANG - NEXTOMICS BIOSCIENCES CO., LTD |
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WANG, DEPENG - NEXTOMICS BIOSCIENCES CO., LTD |
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WANG, FENG - WUHAN UNIVERSITY |
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WANG, LIN - HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY |
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LYON, GHOLSON - COLD SPRING HARBOR LABORATORY |
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GUAN, YONGTAO - CHILDREN'S NUTRITION RESEARCH CENTER (CNRC) |
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SHEN, YUFENG - COLUMBIA UNIVERSITY - NEW YORK |
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EVGRAFOV, OLEG - UNIVERSITY OF SOUTHERN CALIFORNIA |
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KNOWLES, JAMES - UNIVERSITY OF SOUTHERN CALIFORNIA |
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THIBAUD-NISSEN, FRANCOISE - U.S. NATIONAL LIBRARY OF MEDICINE |
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SCHNEIDER, VALERIE - U.S. NATIONAL LIBRARY OF MEDICINE |
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YU, CHACK - THE OHIO STATE UNIVERSITY |
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ZHOU, LIBING - JINAN UNIVERSITY |
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EICHLER, EVAN - UNIVERSITY OF WASHINGTON |
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SO, KWOK - JINAN UNIVERSITY |
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WANG, KAI - UNIVERSITY OF SOUTHERN CALIFORNIA |
Submitted to: Nature Communications
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 5/26/2016 Publication Date: 6/30/2016 Citation: Shi, L., Guo, Y., Dong, C., Huddleston, J., Yang, H., Han, X., Fu, A., Li, Q., Li, N., Gong, S., Lintner, K.E., Ding, Q., Wang, Z., Hu, J., Wang, D., Wang, F., Wang, L., Lyon, G.J., Guan, Y., Shen, Y., Evgrafov, O.V., Knowles, J.A., Thibaud-Nissen, F., Schneider, V., Yu, C.Y., Zhou, L., Eichler, E.E., So, K.F., Wang, K. 2016. Long-read sequencing and de novo assembly of a Chinese genome. Nature Communications. 7:12065. Interpretive Summary: Short-read sequencing has enabled the de novo assembly of several human genomes, but with inherent limitations in characterizing repeat elements. The single-molecule real-time (SMRT) long reads-sequencing technology was used to sequence a Chinese genome. Our results demonstrated the advantage of the long reads technology in genome assembly and gene annotation, and imply that improved characterization of genome functional variation may require the use of a range of genomic technologies on diverse human populations. Technical Abstract: Short-read sequencing has enabled the de novo assembly of several individual human genomes, but with inherent limitations in characterizing repeat elements. Here we sequence a Chinese individual HX1 by single-molecule real-time (SMRT) long-read sequencing, construct a physical map by NanoChannel arrays and generate a de novo assembly of 2.93 Gb (contig N50: 8.3 Mb, scaffold N50: 22.0 Mb, including 39.3 Mb N-bases), together with 206 Mb of alternative haplotypes. The assembly fully or partially fills 274 (28.4%) N-gaps in the reference genome GRCh38. Comparison to GRCh38 reveals 12.8 Mb of HX1-specific sequences, including 4.1 Mb that are not present in previously reported Asian genomes. Furthermore, long-read sequencing of the transcriptome reveals novel spliced genes that are not annotated in GENCODE and are missed by short-read RNA-Seq. Our results imply that improved characterization of genome functional variation may require the use of a range of genomic technologies on diverse human populations. |