Author
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Kindiger, Bryan |
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Submitted to: Plant Molecular Biology International Conference Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 3/8/2017 Publication Date: 6/26/2017 Citation: Kindiger, B.K. 2017. Genome instability in Novel Lolium multiflorum x L. arundinaceum hybrids. Plant Molecular Biology International Conference Proceedings. Available at https://www.eventscribe.com/2017/ASPB/PosterTitles.asp?h=Posters%20and%20Abstracts. Interpretive Summary: Abstract only. Technical Abstract: We have identified a method whereby Lolium multiflorum (Lm) or L. arundinaceum (Fa) genomes are preferentially eliminated through a mitotic loss behavior in interspecific Lm x Fa F1 hybrids,generating either dihaploid Lm lines or Fa lines. The genome instability has been visualized phenotypically and through the use of flow cytometry. The rapid loss of the L. multiflorum “inducer” genome in hybridizations with Fa hybrids has been shown to produce frequent homozygous, dihaploid lines of tall fescue. Flow cytometry, a method for rapidly characterizing optical properties of cells and cell components within individuals, has been applied to identify the prevalence and timing of chimera formation in these Lm x Fa F1hybrids through genome size estimation. Preliminary results indicate flow cytometry can clearly identify genomic sectors within nuclei extracted from leaf tissue, predict the size and frequency of the genomic sectors and can suggest the particular environmental parameters that enhance or reduce chimera formation. Due to the high throughput capabilities of flow cytometry, the method has also been effectively applied toward the rapid identification of both Lm or Fa dihaploid individuals,greatly reducing the need for standard root-tip chromosome squash methods. |
