7,10-Epoxyoctadeca-7,9-dienoic acid: a small molecule adjuvant that potentiates ß-Lactam antibiotics against multidrug-resistant Staphylococcus aureus

Authors: DASAGRANDHI, CHAKRADHAR - Kyungpook National University; KIM, YOUNG-SOON - Korea University; KIM, IN-HWAN - Korea University; Hou, Ching; KIM, HAK-RYUL - Kyungpook National University

Submitted to: Indian Journal of Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/30/2017
Publication Date: 10/9/2017
Citation: Dasagrandhi, C., Kim, Y.S., Kim, I.H., Hou, C.T., Kim, H.R. 2017. 7,10-Epoxyoctadeca-7,9-dienoic Acid: A small molecule adjuvant that potentiates ß-Lactam antibiotics against multidrug-resistant Staphylococcus aureus. Indian Journal of Microbiology. 57(4):461-469. doi: 10.1007/s12088-017-0680-2.
DOI: https://doi.org/10.1007/s12088-017-0680-2

Interpretive Summary: Previously, we discovered a new microbial culture Pseudomonas aeruginosa PR3 that produced 7,10-dihydroxy-fatty acid from oleic acid and trihydroxy fatty acids from linoleic acid. Hydroxy fatty acids are useful as starting materials for the synthesis of specialty chemicals, special military nylon, plastisizers, coating materials, and possibly as physiologically active agents. However,we need an economical process to produce these value-added hydroxyl fatty acid products; ideally, direct from natural oils which contain oleic and linoleic acids in their triacylglycerols. In an effort for economical production of 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) for industrial application, this research was focused on production of DOD from the natural Philippine vegetable nut oils by P. aeruginosa PR3. We first report that pili nut oil could be used as an efficient substrate for the production of DOD by P. aeruginosa PR3. Our findings can facilitate the research on hydroxyl fatty acids production from natural oils such as nut oils by bioprocessing which benefits U.S. consumers.

Technical Abstract: Biocatalytic modification of natural resources can generate novel compounds with specific properties such as higher viscosity and reactivity. Production of hydroxy fatty acid (HFA), originally found in small amounts in plants, is a good example of the biocatalytic modification of natural vegetable oils. HFAs possess high industrial potential in a wide range of applications including resins, waxes, nylons, plastics, lubricants, cosmetics, and additives in coatings and paintings. Recently, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced from oleic acid by Pseudomonas aeruginosa PR3. This study primarily focused on production of DOD by P. aeruginosa PR3 from three natural nut oils, pili nut oil (PNO), palm oil (PO) and virgin coconut oil (VCO) obtained from Philippines. DOD was produced from PNO and PO by strain P. aeruginosa PR3 while PNO was the most efficient substrate among the oils tested. An optimization study for DOD production from PNO revealed that DOD production was maximized at 48h incubation at pH 8.0. Among the carbon sources tested, fructose was efficiently used with a maximum DOD production of 130 mg/50 ml culture while urea was an optimal nitrogen source with a maximum DOD production of 165 mg/50 ml culture. Results from this study demonstrated that PNO could be used as an efficient substrate for the production of DOD by microbial bioconversion.