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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Diet, Genomics and Immunology Laboratory » Research » Publications at this Location » Publication #341748
Title: Absorption, distribution, metabolism and excretion of 3-MCPD 1-monopalmitate after oral administration in rats

Author
item GAO, BOYAN - University Of Maryland
item LIU, MAN - University Of Maryland
item HUANG, GUOREN - Shanghai Jiaotong University
item ZHANG, ZHONGFEI - Beijing Jiaotong University
item ZHAO, YUE - Shanghai Jiaotong University
item Wang, Thomas - Tom
item ZHANG, YAQIONG - Shanghai Jiaotong University
item LIU, JIE - Beijing Advanced Innovation Center For Food Nutrition And Human Health, Beijing Technology & Busine
item YU, LIANGLI - University Of Maryland

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/9/2017
Publication Date: 3/29/2018
Citation: Gao, B., Liu, M., Huang, G., Zhang, Z., Zhao, Y., Wang, T.T., Zhang, Y., Liu, J., Yu, L. 2018. Absorption, distribution, metabolism and excretion of 3-MCPD 1-monopalmitate after oral administration in rats. Journal of Agricultural and Food Chemistry. 65(12):2609-2614. https://doi.org/10.1021/acs.jafc.7b00639.
DOI: https://doi.org/10.1021/acs.jafc.7b00639

Interpretive Summary: Fatty acid esters of monochloropropane 1,2-diol (3-MCPD) are processing-induced toxicants and have been detected in several food categories. This study investigated the absorption, distribution, metabolism and excretion of 3-MCPD esters in Sprague Dawley (SD) rats using 3-MCPD 1-monopalmitate as the probe compound. The kinetics of 3-MCPD 1-monopalmitate in plasma was investigated using (SD) rats, and the results indicated that 3-MCPD 1-monopalmitate was absorbed directly in vivo and metabolized. Its primary metabolites in the liver, kidney, testis, brain, plasma and urine were tentatively identified and measured at 6, 12, 24 and 48 hours after oral administration. Structures were proposed for 8 metabolites. 3-MCPD 1-monopalmitate was converted to free 3-MCPD, which formed the phase II metabolites. All of the metabolites were chlorine-related chemical components; most of them existed in urine, reflecting the excretion pattern of 3-MCPD esters. Understanding the metabolism of 3-MCPD esters in vivo is critical for assessing their toxicities.

Technical Abstract: Fatty acid esters of monochloropropane 1,2-diol (3-MCPD) are processing-induced toxicants and have been detected in several food categories. This study investigated the absorption, distribution, metabolism and excretion of 3-MCPD esters in Sprague Dawley (SD) rats using 3-MCPD 1-monopalmitate as the probe compound. The kinetics of 3-MCPD 1-monopalmitate in plasma was investigated using (SD) rats, and the results indicated that 3-MCPD 1-monopalmitate was absorbed directly in vivo and metabolized. Its primary metabolites in the liver, kidney, testis, brain, plasma and urine were tentatively identified and measured at 6, 12, 24 and 48 hours after oral administration. Structures were proposed for 8 metabolites. 3-MCPD 1-monopalmitate was converted to free 3-MCPD, which formed the phase II metabolites. All the metabolites were chlorine-related chemical components; most of them existed in urine, reflecting the excretion pattern of 3-MCPD esters. Understanding the metabolism of 3-MCPD esters in vivo is critical for assessing their toxicities.