Identification and confirmation of resistance against soybean aphid (Aphis glycines) in eight wild soybean lines

Authors: Hesler, Louis; TILMON, KELLEY - The Ohio State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2017
Publication Date: 11/7/2017
Citation: Hesler, L.S., Tilmon, K. 2017. Identification and confirmation of resistance against soybean aphid (Aphis glycines) in eight wild soybean lines [abstract]. Entomological Society of America meeting, Denver, CO Nov. 5-8, 2017.

Interpretive Summary: The development and use of aphid-resistant soybean cultivars has been complicated by the presence of various resistance-breaking biotypes of the soybean aphid (SA). Ultimately, a variety of unique resistance sources may be needed to develop cultivars with broad spectrum resistance to SA, and those sources may include wild soybean, the ancestor of cultivated soybean. This study screened 339 wild soybean lines against SA biotype 1 in no-cage choice tests, and then further evaluated putatively resistant lines in no-choice cage tests that confined SA to individual lines and measured population growth after 10 and 20 days. Screening tests identified eight lines with significant resistance. All eight lines showed resistance in no-choice tests by limiting SA population growth compared to that on susceptible lines. Four of the eight lines had significantly lower numbers of SA per plant than susceptible lines at both 10 and 20 days of infestation, whereas the other four lines had significantly lower SA numbers than susceptible lines only after 20 days. The identification and confirmation of SA-resistance in eight wild soybean lines in this study is promising, and future studies should genetically characterize these eight lines to determine their usefulness in developing durable SA-resistant soybean cultivars.

Technical Abstract: The development and use of aphid-resistant soybean (Glycine max) cultivars has been complicated by the presence of multiple virulent biotypes of the soybean aphid (SA, Aphis glycines Matsumura). Ultimately, a variety of unique resistance sources may be needed to develop cultivars with a broad spectrum of resistance to SA, and those sources may include wild soybean (Glycine soja), the progenitor of cultivated soybean. This study screened 339 wild soybean lines against SA biotype 1 in no-cage choice tests, and then further evaluated putatively resistant lines in no-choice cage tests that confined SA to individual lines and measured population growth after 10 and 20 days. Screening tests identified eight lines with significant resistance. All eight lines showed resistance in no-choice tests by limiting SA population growth compared to that on susceptible lines. Four of the eight lines had significantly lower numbers of SA per plant than susceptible lines at both 10 and 20 days of infestation, whereas the other four lines had significantly lower SA numbers than susceptible lines only after 20 days. The identification and confirmation of SA-resistance in eight wild soybean lines in this study is promising, and future studies should genetically characterize these eight lines to determine their usefulness in developing durable SA-resistant soybean cultivars.